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http://hdl.handle.net/10261/121981
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Visualizar otros formatos: MARC | Dublin Core | RDF | ORE | MODS | METS | DIDL | DATACITE | |
Campo DC | Valor | Lengua/Idioma |
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dc.contributor.author | Yruela Guerrero, Inmaculada | es_ES |
dc.contributor.author | Kan, P. J .M. van | es_ES |
dc.contributor.author | Müller, Maren | es_ES |
dc.contributor.author | Holzwarth, Alfred R. | es_ES |
dc.date.issued | 1994-02 | - |
dc.identifier.citation | FEBS Letters 339 (1–2): 25–30 (1994) | es_ES |
dc.identifier.issn | 0014-5793 | - |
dc.identifier.uri | http://hdl.handle.net/10261/121981 | - |
dc.description | 6 Pags.- 1 Tabl.- 6 Figs. | es_ES |
dc.description.abstract | A D1-D2-cyt b-559 complex containing 4 chlorophyll a, 1 β-carotene and 1 cytochrome b-559 per 2 pheophytin a has been isolated from spinach with 30% yield using a Q-Sepharose Fast-Flow anion-exchange column equilibrated with 0.1% Triton X-100, 10 mM MgSO4 and 50 mM Tris-HCl (pH 7.2). The preparation was then stabilized with 0.1% dodecyl-β-d-maltoside. This method gave a yield 10 times higher than that using a Fractogel TSK-DEAE 650(S) column equilibrated with 0.1% Triton X-100, 30 mM NaCl and 50 mM Tris-HCl (pH 7.2). The PS II RC complex was characterized using absorption and fluorescence spectroscopy at 277 and 77 K. A selective reversible bleaching under reducing conditions with maximum at 682 nm, associated with pheophytin a reduction, and light-induced absorption differences with a lifetime of 1.0 ms, ascribed to the triplet state of P680 were measured and indicated that the isolated Dl-D2-cyt b-559 complex is active in charge separation. The results are compared with the data obtained for a PS II RC preparation containing 6 chlorophyll a, 2 β-carotene and 1 cyt b-559 per 2 pheophytin a. | es_ES |
dc.language.iso | eng | es_ES |
dc.publisher | Elsevier | es_ES |
dc.rights | closedAccess | es_ES |
dc.subject | Photosystem II | es_ES |
dc.subject | Reaction center | es_ES |
dc.subject | Pigment composition | es_ES |
dc.title | Characterization of a D1-D2-cyt b-559 complex containing 4 chlorophyll a/2 pheophytin a isolated with the use of MgSO4 | es_ES |
dc.type | artículo | es_ES |
dc.identifier.doi | 10.1016/0014-5793(94)80377-3 | - |
dc.description.peerreviewed | Peer reviewed | es_ES |
dc.relation.publisherversion | http://dx.doi.org/10.1016/0014-5793(94)80377-3 | es_ES |
dc.relation.csic | Sí | es_ES |
dc.type.coar | http://purl.org/coar/resource_type/c_6501 | es_ES |
item.languageiso639-1 | en | - |
item.fulltext | No Fulltext | - |
item.openairecristype | http://purl.org/coar/resource_type/c_18cf | - |
item.cerifentitytype | Publications | - |
item.grantfulltext | none | - |
item.openairetype | artículo | - |
Aparece en las colecciones: | (EEAD) Artículos |
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Acceso_Restringido.pdf | 15,38 kB | Adobe PDF | Visualizar/Abrir |
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