English   español  
Please use this identifier to cite or link to this item: http://hdl.handle.net/10261/121923
Share/Impact:
Statistics
logo share SHARE logo core CORE   Add this article to your Mendeley library MendeleyBASE

Visualizar otros formatos: MARC | Dublin Core | RDF | ORE | MODS | METS | DIDL
Exportar a otros formatos:

Title

Direct evidence for the formation of precatenanes during DNA replication.

AuthorsCebrián, Jorge; Castán, Alicia; Martínez, Víctor; Kadomatsu-Hermosa, M.J.; Parra, Cristina; Fernández-Nestosa, María José ; Schaerer, C; Hernandez, Pablo ; Krimer, Dora B. ; Schvartzman, Jorge Bernardo
KeywordsAgarose gel electrophoresis
Bacterial plasmid
Issue Date29-May-2015
PublisherAmerican Society for Biochemistry and Molecular Biology
CitationJournal of Biological Chemistry 290 (22) 13725-13735 (2015)
AbstractThe dynamics of DNA topology during replication are still poorly understood. Bacterial plasmids are negatively supercoiled. This underwinding facilitates strand separation of the DNA duplex during replication. Leading the replisome, a DNA helicase separates the parental strands that are to be used as templates. This strand separation causes overwinding of the duplex ahead. If this overwinding persists, it would eventually impede fork progression. In bacteria, DNA gyrase and topoisomerase IV act ahead of the fork to keep DNA underwound. However, the processivity of the DNA helicase might overcome DNA gyrase and topoisomerase IV. It was proposed that the overwinding that builds up ahead of the fork could force it to swivel and diffuse this positive supercoiling behind the fork where topoisomerase IV would also act to maintain replicating the DNA underwound. Putative intertwining of sister duplexes in the replicated region are called precatenanes. Fork swiveling and the formation of precatenanes, however, are still questioned. Here, we used classical genetics and high resolution two-dimensional agarose gel electrophoresis to examine the torsional tension of replication intermediates of three bacterial plasmids with the fork stalled at different sites before termination. The results obtained indicated that precatenanes do form as replication progresses before termination. © 2015 by The American Society for Biochemistry and Molecular Biology, Inc.
Description11 págs.; 9 figs. este artículo forma parte de tesis doctoral https://digital.csic.es/handle/10261/120925
Publisher version (URL)http://dx.doi.org/10.1074/jbc.M115.642272
URIhttp://hdl.handle.net/10261/121923
DOI10.1074/jbc.M115.642272
E-ISSN00219258
Appears in Collections:(CIB) Artículos
Files in This Item:
File Description SizeFormat 
J. Biol. Chem.-2015-Cebrián-13725-35.pdf2,94 MBAdobe PDFThumbnail
View/Open
Show full item record
Review this work
 

Related articles:


WARNING: Items in Digital.CSIC are protected by copyright, with all rights reserved, unless otherwise indicated.