English   español  
Please use this identifier to cite or link to this item: http://hdl.handle.net/10261/121877
Share/Impact:
Statistics
logo share SHARE logo core CORE   Add this article to your Mendeley library MendeleyBASE

Visualizar otros formatos: MARC | Dublin Core | RDF | ORE | MODS | METS | DIDL | DATACITE
Exportar a otros formatos:

Title

Apoptosis and cell recovery in response to oxidative stress in p53-deficient prostate carcinoma cells

AuthorsBataller, Marc ; Portugal, José
Issue Date15-May-2005
PublisherAcademic Press
CitationArchives of Biochemistry and Biophysics 437(2): 151-158 (2005)
AbstractWe have studied the effects of different concentrations of H 2O2 on the proliferation of PC-3 prostate carcinoma cells. Since this cell line lacks functional p53, we sought to characterize whether apoptotic response to the oxidative insult was altered such that, unlike in cells containing functional p53 apoptosis may be reduced and replaced by other mechanisms of cellular arrest and death. We did not observe necrosis in PC-3 cells treated with H2O2 concentrations of up to 500 μM. In the presence of 50 μM H2O2, arrest was observed in the G2-phase of the cell cycle, along with p53-independent apoptosis. In the presence of 500 μM H2O2, addition of L-buthionine sulfoximine increased the percentage of apoptotic cell death. Senescence-associated cell arrest was never observed. Moreover, some of the treated cells seemed to be resistant to oxidative damage. These cells re-entered the cell cycle and proliferated normally. Analysis of the expression of p21waf1 and of p21 protein levels, as well as the activity of caspase-3 and caspase-8, allowed us to characterize some aspects of the arrest of PC-3 cells in G2 and the apoptotic response to oxidative stress in the absence of functional p53. © 2005 Elsevier Inc. All rights reserved.
Publisher version (URL)http://dx.doi.org/10.1016/j.abb.2005.03.008
URIhttp://hdl.handle.net/10261/121877
DOIhttp://dx.doi.org/10.1016/j.abb.2005.03.008
Identifiersdoi: 10.1016/j.abb.2005.03.008
issn: 0003-9861
Appears in Collections:(IBMB) Artículos
Files in This Item:
File Description SizeFormat 
accesoRestringido.pdf15,38 kBAdobe PDFThumbnail
View/Open
Show full item record
Review this work
 


WARNING: Items in Digital.CSIC are protected by copyright, with all rights reserved, unless otherwise indicated.