English   español  
Por favor, use este identificador para citar o enlazar a este item: http://hdl.handle.net/10261/121873
COMPARTIR / IMPACTO:
Estadísticas
logo share SHARE logo core CORE   Add this article to your Mendeley library MendeleyBASE

Visualizar otros formatos: MARC | Dublin Core | RDF | ORE | MODS | METS | DIDL
Exportar a otros formatos:
Título

A nuclear budding mechanism in transiently arrested cells generates drug-sensitive and drug-resistant cells

AutorMansilla, Sylvia ; Bataller, Marc ; Portugal, José
Fecha de publicación5-abr-2009
EditorElsevier
CitaciónBiochemical Pharmacology 78(2): 123-132 (2009)
ResumenHCT116 (p53+/+) human colon carcinoma cells treated with nanomolar concentrations of doxorubicin underwent transient senescence, synthesized DNA, showed endopolyploidization, increased their size and became multinucleated without a significant increase in mitosis. Nuclei underwent a budding process that involved the release of buds outside the nuclear membrane, and some of the buds seemed to escape from the polyploid cells. A clonogenic assay showed that some cells proliferated following the initial treatment. In general, cells ensuing after budding were not resistant to a variety of drugs, although some of them turned out to be resistant, indicating a potential selective advantage. Nuclear budding was accompanied by changes in protein levels in the giant cells, including inhibition of p53 and enhanced expression of p21WAF1 and the meiosis-related Mos. The buds might be a mechanism for the segregation and elimination of redundant DNA, or for generating viable aneuploid cells with a potentially extended life span. © 2009 Elsevier Inc. All rights reserved.
Versión del editorhttp://dx.doi.org/10.1016/j.bcp.2009.03.027
URIhttp://hdl.handle.net/10261/121873
DOI10.1016/j.bcp.2009.03.027
Identificadoresdoi: 10.1016/j.bcp.2009.03.027
issn: 0006-2952
Aparece en las colecciones: (IBMB) Artículos
Ficheros en este ítem:
Fichero Descripción Tamaño Formato  
accesoRestringido.pdf15,38 kBAdobe PDFVista previa
Visualizar/Abrir
Mostrar el registro completo
 

Artículos relacionados:


NOTA: Los ítems de Digital.CSIC están protegidos por copyright, con todos los derechos reservados, a menos que se indique lo contrario.