English   español  
Por favor, use este identificador para citar o enlazar a este item: http://hdl.handle.net/10261/12176
Compartir / Impacto:
Estadísticas
Add this article to your Mendeley library MendeleyBASE
Visualizar otros formatos: MARC | Dublin Core | RDF | ORE | MODS | METS | DIDL
Título

Separation and characterization of antioxidants from Spirulina platensis microalgacombining pressurized liquid extraction, TLC and HPLC-DAD

AutorJaime, Laura ; Mendiola León, Jose Antonio ; Herrero, Miguel ; Soler-Rivas, Cristina ; Santoyo, Susana ; Señorans, F. Javier; Cifuentes, Alejandro ; Ibáñez, Elena
Palabras claveSpirulina platensis
pressurized liquid extraction
antioxidant activity
TLC
HPLC-DAD
Fecha de publicaciónoct-2005
EditorWiley-VCH
CitaciónJournal of Separation Science, vol. 28, no16, pp. 2111-2119
ResumenA new procedure has been developed to separate and characterize antioxidant compounds from Spirulina platensis microalga based on the combination of pressurized liquid extraction (PLE) and different chromatographic procedures, such as Thin-Layer Chromatography (TLC), at preparative scale, and High Performance Liquid Chromatography (HPLC) with a Diode Array Detector (DAD). Different solvents were tested for PLE extraction of antioxidants from Spirulina platensis microalga. An optimized pressurized liquid extraction process using ethanol (generally recognized as safe, GRAS) as extraction solvent has been obtained that provides natural extracts with high yields and good antioxidant properties. TLC analysis of this ethanolic extract obtained at 115°C for 15 min was carried out and the silica layer was stained with a DPPH• (diphenyl-pycril-hydrazyl) radical solution to determine the antioxidant activity of the different chromatographic bands. Next, these coloured bands were collected for their subsequent analysis by HPLC-DAD revealing that the compounds with the most important antioxidant activity present in Spirulina extracts were carotenoids, as well as phenolic compounds and degradation products of chlorophylls.
Versión del editorhttp://dx.doi.org/10.1002/jssc.200500185
URIhttp://hdl.handle.net/10261/12176
DOI10.1002/jssc.200500185
ISSN1615-9306
Aparece en las colecciones: (IFI) Artículos
Ficheros en este ítem:
Fichero Descripción Tamaño Formato  
JSepSci 28 (2005) 2111-2119.pdf799,51 kBAdobe PDFVista previa
Visualizar/Abrir
Mostrar el registro completo
 

Artículos relacionados:


NOTA: Los ítems de Digital.CSIC están protegidos por copyright, con todos los derechos reservados, a menos que se indique lo contrario.