Please use this identifier to cite or link to this item:
logo share SHARE logo core CORE BASE
Visualizar otros formatos: MARC | Dublin Core | RDF | ORE | MODS | METS | DIDL | DATACITE

5-hydroxymethylfurfural conversion by fungal aryl-alcohol oxidase and unspecific peroxygenase

AuthorsCarro, Juan ; Ferreira, Patricia CSIC ORCID; Rodríguez, Leonor; Prieto, Alicia CSIC ORCID ; Serrano, Ana; Balcells, Beatriz; Ardá, Ana CSIC ORCID CVN; Jiménez-Barbero, Jesús CSIC ORCID; Gutiérrez Suárez, Ana CSIC ORCID ; Martínez, Ángel T. CSIC ORCID
Keywords2,5-formylfurancarboxylic acid
2,5-furandicarboxylic acid
Aryl-alcohol oxidase
Unspecific peroxygenase
Issue Date8-Jan-2015
PublisherJohn Wiley & Sons
CitationFEBS Journal 282: 3218-3229 (2014)
AbstractOxidative conversion of 5-hydroxymethylfurfural (HMF) is of biotechnological interest for the production of renewable (lignocellulose-based) platform chemicals, such as 2,5-furandicarboxylic acid (FDCA). To the best of our knowledge, the ability of fungal aryl-alcohol oxidase (AAO) to oxidize HMF is reported here for the first time, resulting in almost complete conversion into 2,5-formylfurancarboxylic acid (FFCA) in a few hours. The reaction starts with alcohol oxidation, yielding 2,5-diformylfuran (DFF), which is rapidly converted into FFCA by carbonyl oxidation, most probably without leaving the enzyme active site. This agrees with the similar catalytic efficiencies of the enzyme with respect to oxidization of HMF and DFF, and its very low activity on 2,5-hydroxymethylfurancarboxylic acid (which was not detected by GC-MS). However, AAO was found to be unable to directly oxidize the carbonyl group in FFCA, and only modest amounts of FDCA are formed from HMF (most probably by chemical oxidation of FFCA by the H2O2 previously generated by AAO). As aldehyde oxidation by AAO proceeds via the corresponding geminal diols (aldehyde hydrates), the various carbonyl oxidation rates may be related to the low degree of hydration of FFCA compared with DFF. The conversion of HMF was completed by introducing a fungal unspecific heme peroxygenase that uses the H2O2 generated by AAO to transform FFCA into FDCA, albeit more slowly than the previous AAO reactions. By adding this peroxygenase when FFCA production by AAO has been completed, transformation of HMF into FDCA may be achieved in a reaction cascade in which O2 is the only co-substrate required, and water is the only by-product formed. © 2014 The Authors. FEBS Journal published by John Wiley & Sons Ltd on behalf of FEBS.
Description12 páginas.-- 6 figuras.-- 2 tablas.-- 34 referencias.-- Additional supporting information may be found in the online version of this article at the publisher’s web site: Fig. S1. GC-MS analysis of standard compounds. Fig. S2. 1H-NMR spectra for HMF, DFF and FFCA in DMSO-d6. Table S1. Kinetic constants for a variety of AAO substrates. Doc. S1. MS identification of products from HMF conversion into FDCA. FEBS
Publisher version (URL)
Appears in Collections:(CIB) Artículos
(IRNAS) Artículos

Files in This Item:
File Description SizeFormat
Carro_et_al-2015-FEBS_Journal.pdf853,52 kBAdobe PDFThumbnail
Show full item record
Review this work

PubMed Central

checked on May 13, 2022


checked on May 13, 2022


checked on May 15, 2022

Page view(s)

checked on May 16, 2022


checked on May 16, 2022

Google ScholarTM




Related articles:

This item is licensed under a Creative Commons License Creative Commons