English   español  
Please use this identifier to cite or link to this item: http://hdl.handle.net/10261/121345
Share/Impact:
Statistics
logo share SHARE logo core CORE   Add this article to your Mendeley library MendeleyBASE

Visualizar otros formatos: MARC | Dublin Core | RDF | ORE | MODS | METS | DIDL
Exportar a otros formatos:
Title

Cell compatibility of a maghemite/polymer biomedical nanoplatform

AuthorsAli, L. M. A.; Piñol, Rafael; Villa-Bellosta, Ricardo; Gabilondo, Lierni; Millán, Ángel; Palacio, Fernando; Sorribas, Víctor
KeywordsIron oxide
Nanoparticles
Cytotoxicity
Subcellular localization
Cell internalization
Issue DateApr-2015
PublisherElsevier
CitationToxicology in Vitro 29(5): 962-975 (2015)
AbstractWe are reporting the cytocompatibility and cellular fate of an iron oxide/polymer nanoplatform (IONP) in its most basic formulation, using both mesenchymal (vascular smooth muscle cells, VSMC), and epithelial (opossum kidney, OK) cells. The cytotoxicity and cell internalization of the nanoplatform has been evaluated in relation to time of exposure and concentration of different components. A series of samples with different iron oxide nanoparticle, sizes, hydrodynamic sizes and iron/polymer ratio have been examined. In all cases cytotoxicity is low, and it is mostly determined by the internalization rate, being higher in VSMC than in OK cells. The mean lethal dose has a very narrow threshold, and necrosis is the only cell death type. IONP uptake shows little incidence on oxidative stress, and inflammasome activation is only observed with the smaller IONP at high concentration. The internalization rate in VSMC is determined by the polymer concentration exclusively. In OK cells, internalization rate seems to increase with decreasing hydrodynamic size. Internalization occurs through clathrin-dependent endocytosis, as it is prevented by potassium depletion and chlorpromazine. IONP are directed and accumulated in lysosomes. Under IONP overload, lysosomal dysfunction would cause cell death using concentrations that are hardly achieved in vivo.
Publisher version (URL)http://dx.doi.org/10.1016/j.tiv.2015.04.003
URIhttp://hdl.handle.net/10261/121345
DOI10.1016/j.tiv.2015.04.003
ISSN0887-2333
E-ISSN1879-3177
Appears in Collections:(ICMA) Artículos
Files in This Item:
File Description SizeFormat 
accesoRestringido.pdf15,38 kBAdobe PDFThumbnail
View/Open
Show full item record
Review this work
 

Related articles:


WARNING: Items in Digital.CSIC are protected by copyright, with all rights reserved, unless otherwise indicated.