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Título

ATP binding on a mutant of OleB', the first half of an ABC transporter of Streptomyces antibioticus

AutorBuche, André ; Aparicio, G.; Méndez, Carmen; Salas, J. A.
Fecha de publicación1998
CitaciónJournal of Biochemistry, Molecular Biology and Biophysics 2: 7-11 (1998)
ResumenOleB' is the first half of an oleandomycin ABC transporter of Streptomyces antibioticus. This protein contains a conserved Lys in the well known 'Walker A' motif. For several ABC transporters it has been shown that mutation of this amino acid alters the transport process indicating that this amino acid has a specific role in at least one of the different phenomena regulating transport (ATP binding, hydrolysis, structural modification or substrate binding). In this work we observed that substitution of Lys 71 of Walker A motif by a Gly does not change drastically the ability of ATP to bind to OleB' as observed by the extrinsic fluorescence of TNP-ATP and by competition between TNP-ATP and ATP for the binding site.
URIhttp://hdl.handle.net/10261/121284
DOInull
Identificadoresdoi: null
issn: 1025-8140
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