English   español  
Por favor, use este identificador para citar o enlazar a este item: http://hdl.handle.net/10261/121272
logo share SHARE logo core CORE   Add this article to your Mendeley library MendeleyBASE

Visualizar otros formatos: MARC | Dublin Core | RDF | ORE | MODS | METS | DIDL
Exportar a otros formatos:

Bicarbonate is an essential constituent of the water-oxidizing complex of photosystem II

AutorAllakhverdiev, Suleyman I.; Yruela Guerrero, Inmaculada ; Picorel Castaño, Rafael ; Klimov, Vyacheslav V.
Fecha de publicación1997
EditorNational Academy of Sciences (U.S.)
CitaciónProceedings of the National Academy of Sciences of the United States of America 94: 5050-5054 (1997)
ResumenIt is shown that restoration of photoinduced electron flow and O2 evolution with Mn2+ in Mn-depleted photosystem II (PSII) membrane fragments isolated from spinach chloroplasts is considerably increased with bicarbonate in the region pH 5.0-8.0 in bicarbonate-depleted medium. In buffered solutions equilibrated with the atmosphere (nondepleted of bicarbonate), the bicarbonate effect is observed only at pH lower than the pK of H2CO3 dissociation (6.4), which indicates that HCO3/- is the essential species for the restoration effect. The addition of just 2 Mn2+ atoms per one PSII reaction center is enough for the maximal reactivation when bicarbonate is present in the medium. Analysis of bicarbonate concentration dependence of the restoration effect reveals two binding sites for bicarbonate with apparent dissociation constant (K(d)) of ≃2.5 μM and 20-34 μM when 2,6- dichloro-p-benzoquinone is used as electron acceptor, while in the presence of silicomolybdate only the latter one remains. Similar bicarbonate concentration dependence of O2 evolution was obtained in untreated Mn- containing PSII membrane fragments. It is suggested that the K(d) of 20-34 μM is associated with the donor side of PSII while the location of the lower K(d) binding site is not quite clear. The conclusion is made that bicarbonate is an essential constituent of the water-oxidizing complex of PSII, important for its assembly and maintenance in the functionally active state.
Identificadoresdoi: 10.1073/pnas.94.10.5050
issn: 0027-8424
Aparece en las colecciones: (EEAD) Artículos
Ficheros en este ítem:
Fichero Descripción Tamaño Formato  
accesoRestringido.pdf15,38 kBAdobe PDFVista previa
Mostrar el registro completo

Artículos relacionados:

NOTA: Los ítems de Digital.CSIC están protegidos por copyright, con todos los derechos reservados, a menos que se indique lo contrario.