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New ionic bis-MPA and PAMAM dendrimers: A study of their biocompatibility and DNA-complexation

AuthorsMovellan, Julie; Fuente, Jesús M. de la; Martín-Duque, Pilar; Serrano, José Luis; Sierra, Teresa
Biological carriers
Issue DateMay-2015
CitationMacromolecular Bioscience 15(5): 657-667 (2015)
AbstractHerein, the synthesis of five novel ionic dendrimers and their evaluation as biological carriers is reported. The compounds include an ionic bis-MPA dendrimer and four PAMAM dendrimers of different generations decorated with negatively charged hydrophilic chains of 2-[2-(2-methoxyethoxy)ethoxy]acetic acid as counter ions in order to increase their biocompatibility. The ionic dendrimers derived from bis-MPA have a low cytotoxicity at 0.5 mg · mL−1 against U251MG and are even less toxic against mesenchymal stem cells; however, the PAMAM derivatives show high cytotoxicity at the same concentration. The five compounds are able to form complexes with plasmid DNA at different N/P ratios. The cytotoxicity and complexation ability of the new dendrimers were also compared with jetPEI, a linear polyethylenimine derivative commercially available as transfection reagent. The results indicate that the cytotoxicity of the ionic PAMAM dendrimers remains as an important drawback, whereas the ionic I-bis-MPA compound exhibits a high ability to complex pDNA and very low toxicity compared with jetPEI.
Descriptionet al.
Publisher version (URL)http://dx.doi.org/10.1002/mabi.201400422
Appears in Collections:(ICMA) Artículos
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