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Por favor, use este identificador para citar o enlazar a este item: http://hdl.handle.net/10261/121230
Título

RNAi-based glyconanoparticles trigger apoptotic pathways for in vitro and in vivo enhanced cancer-cell killing

AutorConde, João; Tian, Furong; Hernandez, Yulán; Bao, Chenchen; Baptista, Pedro V.; Cui, Daxiang; Stoeger, Tobias; Fuente, Jesús M. de la
Fecha de publicaciónabr-2015
EditorRoyal Society of Chemistry (Great Britain)
CitaciónNanoscale 7(19): 9083-9091 (2015)
ResumenGold glyconanoparticles (GlycoNPs) are full of promise in areas like biomedicine, biotechnology and materials science due to their amazing physical, chemical and biological properties. Here, siRNA GlycoNPs (AuNP@PEG@Glucose@siRNA) in comparison with PEGylated GlycoNPs (AuNP@PEG@Glucose) were applied in vitro to a luciferase-CMT/167 adenocarcinoma cancer cell line and in vivo via intratracheal instillation directly into the lungs of B6 albino mice grafted with luciferase-CMT/167 adenocarcinoma cells. siRNA GlycoNPs but not PEGylated GlycoNPs induced the expression of pro-apoptotic proteins such as Fas/CD95 and caspases 3 and 9 in CMT/167 adenocarcinoma cells in a dose dependent manner, independent of the inflammatory response, evaluated by bronchoalveolar lavage cell counting. Moreover, in vivo pulmonary delivered siRNA GlycoNPs were capable of targeting c-Myc gene expression (a crucial regulator of cell proliferation and apoptosis) via in vivo RNAi in tumour tissue, leading to an ∼80% reduction in tumour size without associated inflammation.
DescripciónThis article is licensed under a Creative Commons Attribution 3.0 Unported Licence.
Versión del editorhttp://dx.doi.org/10.1039/C4NR05742B
URIhttp://hdl.handle.net/10261/121230
DOI10.1039/C4NR05742B
ISSN2040-3364
E-ISSN2040-3372
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