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Por favor, use este identificador para citar o enlazar a este item: http://hdl.handle.net/10261/121178
Título

Medicago truncatula Natural Resistance-Associated Macrophage Protein1 Is Required for Iron Uptake by Rhizobia-Infected Nodule Cells

AutorTejada-Jiménez, Manuel; Castro-Rodríguez, Rosario; Kryvoruchko, Igor; Lucas, M. Mercedes ; Udvardi, Michael; Imperial, Juan; González-Guerrero, Manuel
Palabras claveMedicago truncatula
Nodule Cells
Macrophage Protein
Iron
symbiotic nitrogen fixation (SNF)
apoplast
rhizobia-infected cells
Fecha de publicaciónmay-2015
EditorAmerican Society of Plant Biologists
CitaciónPlant Physiology, 168: 258–272 (2015)
ResumenIron is critical for symbiotic nitrogen fixation (SNF) as a key component of multiple ferroproteins involved in this biological process. In the model legume Medicago truncatula, iron is delivered by the vasculature to the infection/maturation zone (zone II) of the nodule, where it is released to the apoplast. From there, plasma membrane iron transporters move it into rhizobia-containing cells, where iron is used as the cofactor of multiple plant and rhizobial proteins (e.g. plant leghemoglobin and bacterial nitrogenase). MtNramp1 (Medtr3g088460) is the M. truncatula Natural Resistance-Associated Macrophage Protein family member, with the highest expression levels in roots and nodules. Immunolocalization studies indicate that MtNramp1 is mainly targeted to the plasma membrane. A loss-of-function nramp1 mutant exhibited reduced growth compared with the wild type under symbiotic conditions, but not when fertilized with mineral nitrogen. Nitrogenase activity was low in the mutant, whereas exogenous iron and expression of wild-type MtNramp1 in mutant nodules increased nitrogen fixation to normal levels. These data are consistent with a model in which MtNramp1 is the main transporter responsible for apoplastic iron uptake by rhizobia-infected cells in zone II.
Descripción30 páginas, 9 figuras, 1 tabla, 13 páginas de explicaciones suplementarias de las figuras, más otras 10 figuras suplementarias,
Versión del editorwww.plantphysiol.org/cgi/doi/10.1104/pp.114.254672
URIhttp://hdl.handle.net/10261/121178
DOI10.1104/pp.114.254672
ISSN0032-0889
E-ISSN1532-2548
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