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Título

Production of gynogenic haploids of Hordeum vulgare L.

AutorCastillo Alonso, Ana María ; Cistué Sola, Luis
Fecha de publicación1993
EditorSpringer
CitaciónPlant Cell Reports 12: 139-143 (1993)
ResumenHaploid plants were regenerated from cultured unfertilized ovaries of Hordeum vulgare L. (barley). Optimal response was obtained by the addition of 0.6 μM 4-chloro-2-methylphenoxyacetic acid (MCPA), 2.8 μM indole-3-acetic acid (IAA) and 4.4 μM 6-benzyladenine (BA) in the N6 medium. Further increase in the rate of callus formation and the number of green plants produced was possible with the addition of 90 g/l sucrose and 100 g/l coconut water. The stage of development of the ovaries at the time of culture was critical; the largest number of plants being produced by ovaries from flowers at the trinucleate stage of pollen.
URIhttp://hdl.handle.net/10261/121141
DOI10.1007/BF00239094
Identificadoresdoi: 10.1007/BF00239094
issn: 0721-7714
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