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Título

Detection of genetically modified maize by PCR and capillary gel electrophoresis with UV detection and laser induced fluorescence

AutorGarcía-Cañas, Virginia ; González García, Ramón ; Cifuentes, Alejandro
Palabras claveDNA
capillary gel electrophoresis
polymerase chain reaction
transgenic maize
Bt maize
GMOs
UV
LIF
Fecha de publicación24-ene-2002
EditorAmerican Chemical Society
CitaciónJ. Agric. Food Chem., 2002, 50 (5), pp 1016–1021
ResumenIn this paper, the possibilities of capillary gel electrophoresis (CGE) to detect transgenic maize in flours are shown. The method is based on the extraction and amplification by the polymerase chain reaction (PCR) of a specific DNA fragment from transgenic maize and its subsequent analysis by CGE with UV detection or laser-induced fluorescence (LIF). Some useful considerations regarding the optimization of DNA extraction and amplification conditions are given. Also, a comparison is established between the two CGE protocols for DNA detection based on ultraviolet absorption (CGE-UV) and LIF (CGE-LIF). The requirements, advantages, and limitations of both CGE methods are discussed. To our knowledge, this is the first paper on the use of CGE-LIF to detect transgenic food.
Versión del editorhttp://pubs.acs.org/doi/abs/10.1021/jf011033g
URIhttp://hdl.handle.net/10261/12110
DOI10.1021/jf011033g
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