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Title

Detection of genetically modified maize by PCR and capillary gel electrophoresis with UV detection and laser induced fluorescence

AuthorsGarcía-Cañas, Virginia CSIC ORCID ; González García, Ramón CSIC ORCID ; Cifuentes, Alejandro CSIC ORCID
KeywordsDNA
Capillary gel electrophoresis
polymerase chain reaction
transgenic maize
Bt maize
GMOs
UV
LIF
Issue Date24-Jan-2002
PublisherAmerican Chemical Society
CitationJ. Agric. Food Chem., 2002, 50 (5), pp 1016–1021
AbstractIn this paper, the possibilities of capillary gel electrophoresis (CGE) to detect transgenic maize in flours are shown. The method is based on the extraction and amplification by the polymerase chain reaction (PCR) of a specific DNA fragment from transgenic maize and its subsequent analysis by CGE with UV detection or laser-induced fluorescence (LIF). Some useful considerations regarding the optimization of DNA extraction and amplification conditions are given. Also, a comparison is established between the two CGE protocols for DNA detection based on ultraviolet absorption (CGE-UV) and LIF (CGE-LIF). The requirements, advantages, and limitations of both CGE methods are discussed. To our knowledge, this is the first paper on the use of CGE-LIF to detect transgenic food.
Publisher version (URL)http://pubs.acs.org/doi/abs/10.1021/jf011033g
URIhttp://hdl.handle.net/10261/12110
DOI10.1021/jf011033g
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