English   español  
Please use this identifier to cite or link to this item: http://hdl.handle.net/10261/12110
Share/Impact:
Statistics
logo share SHARE logo core CORE   Add this article to your Mendeley library MendeleyBASE

Visualizar otros formatos: MARC | Dublin Core | RDF | ORE | MODS | METS | DIDL | DATACITE
Exportar a otros formatos:

Title

Detection of genetically modified maize by PCR and capillary gel electrophoresis with UV detection and laser induced fluorescence

AuthorsGarcía-Cañas, Virginia ; González García, Ramón ; Cifuentes, Alejandro
KeywordsDNA
capillary gel electrophoresis
polymerase chain reaction
transgenic maize
Bt maize
GMOs
UV
LIF
Issue Date24-Jan-2002
PublisherAmerican Chemical Society
CitationJ. Agric. Food Chem., 2002, 50 (5), pp 1016–1021
AbstractIn this paper, the possibilities of capillary gel electrophoresis (CGE) to detect transgenic maize in flours are shown. The method is based on the extraction and amplification by the polymerase chain reaction (PCR) of a specific DNA fragment from transgenic maize and its subsequent analysis by CGE with UV detection or laser-induced fluorescence (LIF). Some useful considerations regarding the optimization of DNA extraction and amplification conditions are given. Also, a comparison is established between the two CGE protocols for DNA detection based on ultraviolet absorption (CGE-UV) and LIF (CGE-LIF). The requirements, advantages, and limitations of both CGE methods are discussed. To our knowledge, this is the first paper on the use of CGE-LIF to detect transgenic food.
Publisher version (URL)http://pubs.acs.org/doi/abs/10.1021/jf011033g
URIhttp://hdl.handle.net/10261/12110
DOIhttp://dx.doi.org/10.1021/jf011033g
Appears in Collections:(IFI) Artículos
Files in This Item:
File Description SizeFormat 
dummy_file.txt88 BTextView/Open
Show full item record
Review this work
 


WARNING: Items in Digital.CSIC are protected by copyright, with all rights reserved, unless otherwise indicated.