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Two routes of metabolic cross-feeding between Bifidobacterium adolescentis and butyrate-producing anaerobes from the human gut

AutorBelenguer, Álvaro ; Duncan, Sylvia H.; Calder, A. Graham; Holtrop, Grietje; Louis, P.; Lobley, Gerald E.; Flint, Harry J.
Palabras claveHuman large-intestine
Real-time PCR
Human colon
Resistant starch
Human feces
Microbial communities
Fecal microbiota
Extensive set
Fecha de publicación2006
EditorAmerican Society for Microbiology
CitaciónApplied and Environmental Microbiology 72(5): 3593-3599 (2006)
ResumenDietary carbohydrates have the potential to influence diverse functional groups of bacteria within the human large intestine. Of 12 Bifidobacterium strains of human gut origin from seven species tested, four grew in pure culture on starch and nine on fructo-oligosaccharides. The potential for metabolic cross-feeding between Bifidobacterium adolescentis and lactate-utilizing, butyrate-producing Firmicute bacteria related to Eubacterium hallii and Anaerostipes caccae was investigated in vitro. E. hallii L2-7 and A. caccae L1-92 failed to grow on starch in pure culture, but in coculture with B. adolescentis L2-32 butyrate was formed, indicating cross-feeding of metabolites to the lactate utilizers. Studies with [(13)C]lactate confirmed carbon flow from lactate, via acetyl coenzyme A, to butyrate both in pure cultures of E. hallii and in cocultures with B. adolescentis. Similar results were obtained in cocultures involving B. adolescentis DSM 20083 with fructo-oligosaccharides as the substrate. Butyrate formation was also stimulated, however, in cocultures of B. adolescentis L2-32 grown on starch or fructo-oligosaccharides with Roseburia sp. strain A2-183, which produces butyrate but does not utilize lactate. This is probably a consequence of the release by B. adolescentis of oligosaccharides that are available to Roseburia sp. strain A2-183. We conclude that two distinct mechanisms of metabolic cross-feeding between B. adolescentis and butyrate-forming bacteria may operate in gut ecosystems, one due to consumption of fermentation end products (lactate and acetate) and the other due to cross-feeding of partial breakdown products from complex substrates.
Descripción7 pages, 4 tables, 4 figures.-- PMID: 16672507 [PubMed]
Versión del editorhttp://dx.doi.org/10.1128/AEM.72.5.3593-3599.2006
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