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Título: | From a Short Amino Acidic Sequence to the Complete Gene |
Autor: | Miñambres Rodríguez, Baltasar CSIC; Olivera, Elías R.; García, Belén; Naharro, Germán; Luengo, José M. | Fecha de publicación: | 2000 | Editor: | Elsevier | Citación: | Biochemical and Biophysical Research Communications 272(2): 477-479 (2000) | Resumen: | A useful strategy directed to the isolation of a required gene with a high GC content is reported. Using a degenerate oligonucleotide probe, deduced from the amino terminus of a protein, it is possible to obtain a fragment of DNA containing its encoding gene by PCR amplification. Furthermore, the cloning of a desired gene can be accomplished in two steps by using an oligonucleotide deduced (i) from an internal sequence, (ii) from a consensus sequence, or (iii) from a DNA sequence adjacent to a disrupting element (transposon, insertion sequence, cassette). This method, which could be applied to a bacteriophage, plasmid, or cosmid genomic library, has been successfully used for cloning several genes from different biological systems. | Descripción: | 3 pages, 1 figure.-- PMID: 10833438 [PubMed]. | Versión del editor: | http://dx.doi.org/10.1006/bbrc.2000.2709 | URI: | http://hdl.handle.net/10261/12024 | DOI: | 10.1006/bbrc.2000.2709 | ISSN: | 0006-291X |
Aparece en las colecciones: | (IGM) Artículos |
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