Please use this identifier to cite or link to this item: http://hdl.handle.net/10261/12006
Share/Export:
logo share SHARE BASE
Visualizar otros formatos: MARC | Dublin Core | RDF | ORE | MODS | METS | DIDL | DATACITE
Title

The aryl hydrocarbon receptor repressor

AuthorsZudaire, Enrique; Cuesta, Natalia CSIC ORCID CVN; Murty, Vundavalli; Woodson, Karen; Adams, Lisa; González, Nieves; Martínez, Alfredo; Narayan, Gopeshwar; Kirsch, Ilan; Franklin, Wilbur; Hirsch, Fred; Birrer, Michael; Cuttitta, F.
KeywordsCancer
Issue DateFeb-2008
PublisherAmerican Society for Clinical Investigation
CitationJournal of Clinical Investigation 2008; vol. 118 (2): 640-650
AbstractThe aryl hydrocarbon receptor repressor (AHRR) is a bHLH/Per-ARNT-Sim transcription factor located in a region of chromosome 5 (5p15.3) that has been proposed to contain one or more tumor suppressor genes. We report here consistent downregulation of AHRR mRNA in human malignant tissue from different anatomical origins, including colon, breast, lung, stomach, cervix, and ovary, and demonstrate DNA hypermethylation as the regulatory mechanism of AHRR gene silencing. Knockdown of AHRR gene expression in a human lung cancer cell line using siRNA significantly enhanced in vitro anchorage-dependent and -independent cell growth as well as cell growth after transplantation into immunocompromised mice. In addition, knockdown of AHRR in non-clonable normal human mammary epithelial cells enabled them to grow in an anchorage-independent manner. Further, downregulation of AHRR expression in the human lung cancer cell line conferred resistance to apoptotic signals and enhanced motility and invasion in vitro and angiogenic potential in vivo. Ectopic expression of AHRR in tumor cells resulted in diminished anchorage-dependent and -independent cell growth and reduced angiogenic potential. These results therefore demonstrate that AHRR is a putative new tumor suppressor gene in multiple types of human cancers.
Publisher version (URL)http://www.jci.org/118/2
URIhttp://hdl.handle.net/10261/12006
ISSN0021-9738
Appears in Collections:(IC) Artículos

Files in This Item:
File Description SizeFormat
MartinezAlfredo.pdf847,52 kBAdobe PDFThumbnail
View/Open
Show full item record
Review this work

Page view(s)

300
checked on May 17, 2022

Download(s)

188
checked on May 17, 2022

Google ScholarTM

Check


WARNING: Items in Digital.CSIC are protected by copyright, with all rights reserved, unless otherwise indicated.