Please use this identifier to cite or link to this item: http://hdl.handle.net/10261/11990
Share/Export:
logo share SHARE logo core CORE BASE
Visualizar otros formatos: MARC | Dublin Core | RDF | ORE | MODS | METS | DIDL | DATACITE
Title

Multi-way analysis for investigation of industrial pectin using an analytical liquid dilution system

AuthorsZachariassen, Christian B.; Larsen, Jan; Van den Berg, Frans; Bro, Rasmus; Juan, Anna de; Tauler, Romà
Issue Date3-Jul-2006
PublisherElsevier
CitationChemometric and Intelligent Laboratory Systems 84(1-2): 9-20 (2006)
AbstractMeasurements from an analytical liquid dilution system are used to quantify the intra-molecular distribution of ester groups on the pectin carbohydrate backbone. Thirty-one pectins have been produced from remethylated pectin by enzymatically deesterifying them in steps to give different known ester distributions. The amount of deesterification is measured in each step by titration to provide the reference values. The system works by injecting a solution of pectin into a carrier stream containing a fixed concentration of dye. The dye binds site-specifically to the poly-α-(1 → 4)-D-galacturonic acids constituting the non-esterified parts of the pectin carbohydrate backbone. The carrier stream is led to a Continuously Stirred Tank Reactor (CSTR). The pectin is slowly diluted while UV–VIS spectra are recorded at the reactor outlet providing a landscape of wavelength-by-time for every sample. All pectins are measured this way in triplicate runs.
In an article preceding this, the acquired landscapes have been analysed qualitatively using Multivariate Curve Resolution (MCR) and PARAFAC2, both Alternating Least Squares (ALS) regression algorithms. It is concluded that the landscapes can be described by common spectral profiles for all pectins and individual concentration/time profiles for each sample run.
In this article, calibration to the reference values are done by multi-way Partial Least Squares (PLS) regression to correlate the acquired landscapes as independent variables directly to the reference values as dependent variables. Also, calibration is done by unfolding the landscapes for each sample run to a vector and use conventional PLS. The concentration/time profiles previously identified by MCR-ALS or PARAFAC2 are unfolded and used as independent variables in PLS rather than the whole landscape. Finally, the spectral information can be reduced even further by summing up or integrating the mentioned individual concentration profiles to just one number per profile identified by MCR-ALS or PARAFAC2 in the sample run, or using the identified score values for each profile from the PARAFAC2 model as new independent variables.
The most successful calibration models based on a calibration set built from one of the triplicates can predict the induced degree of deesterification to an error level of less than 3% in absolute values–corresponding to a 6% relative error to the calibration full range–when tested onto a set consisting of the two remaining sample runs from the full set. The best calibration models are based either on unfolded landscapes or unfolded concentration profiles resolved by MCR-ALS.
Description12 pages, 7 figures, 2 tables.-- Printed version published Dec 2006.-- Issue title: "Selected papers presented at the 9th Scandinavian Symposium on Chemometrics (SSC9, Reykjavik, Iceland, Aug 21–25, 2005)".
Publisher version (URL)http://dx.doi.org/10.1016/j.chemolab.2006.03.010
URIhttp://hdl.handle.net/10261/11990
DOI10.1016/j.chemolab.2006.03.010
ISSN0169-7439
Appears in Collections:(IDAEA) Artículos

Show full item record
Review this work

SCOPUSTM   
Citations

7
checked on Jan 12, 2022

WEB OF SCIENCETM
Citations

6
checked on Jan 18, 2022

Page view(s)

450
checked on Jan 20, 2022

Google ScholarTM

Check

Altmetric

Dimensions


WARNING: Items in Digital.CSIC are protected by copyright, with all rights reserved, unless otherwise indicated.