English   español  
Please use this identifier to cite or link to this item: http://hdl.handle.net/10261/11921
Share/Impact:
Statistics
logo share SHARE logo core CORE   Add this article to your Mendeley library MendeleyBASE

Visualizar otros formatos: MARC | Dublin Core | RDF | ORE | MODS | METS | DIDL
Exportar a otros formatos:

Title

Identification of novel glucocorticoid receptor-regulated genes involved in epidermal homeostasis and hair follicle differentiation

AuthorsDonet, Eva; Bayo, Pilar ; Calvo, Ezequiel; Labrie, Fernand; Pérez, Paloma
Keywordsglucocorticoid receptor
epidermal homeostasis
microarray analysis
transgenic mice
Issue Date7-Sep-2007
PublisherElsevier
CitationThe Journal of Steroid Biochemistry and Molecular Biology 108 (1-2): 8-16 (2008)
AbstractDespite that glucocorticoids (GCs), acting through the glucocorticoid receptor (GR) exert a pivotal role in skin physiopathology, specific genes regulated by GR in this tissue are largely unknown. We have used a transgenic mouse model overexpressing GR in epidermal basal cells and outer root sheath (ORS) of the hair follicle (HF) under the control of the keratin 5 regulatory sequences (K5-GR mice) to identify GR-regulated genes in mouse skin. We analyzed the transcriptomic profile of adult K5-GR skin as compared to non-transgenic adult mice by using oligonucleotide microarrays and identified 173 genes differentially regulated by GR in this tissue. Our data were further validated by semiquantitative RT-PCR and quantitative real-time PCR. We have identified a large subset of hair keratin intermediate filament (krt) and hair keratin-associated protein (krtap) genes, as well as several hox genes as GC-regulated. Since dysregulation of krt, krtaps and hox genes can cause hair disorders, as it occurs in adult K5-GR mice, our findings strongly suggest a role of GR in HF morphogenesis through the coordinated regulation of these hair-specific genes. In addition, we found that GR repressed several genes related to cell growth, such as the immediate early genes fosb and c-fos, according to the antiproliferative role described for this hormone receptor. By using cultured keratinocytes treated with GR-agonists and -antagonists, we demonstrated that down-regulation of fosb is mediated by GR. Identification of novel GR-regulated genes will help us to better understand the role of GCs as physiological modulators and pharmacological agents.
Description9 pages, 3 figures PMID: 17935973 [PubMed]
Publisher version (URL)http://dx.doi.org/10.1016/j.jsbmb.2007.05.033
URIhttp://hdl.handle.net/10261/11921
DOI10.1016/j.jsbmb.2007.05.033
ISSN0960-0760
Appears in Collections:(IBV) Artículos
Files in This Item:
File Description SizeFormat 
Donet JSBMB 07.pdf2,19 MBAdobe PDFThumbnail
View/Open
Show full item record
Review this work
 

Related articles:


WARNING: Items in Digital.CSIC are protected by copyright, with all rights reserved, unless otherwise indicated.