English   español  
Por favor, use este identificador para citar o enlazar a este item: http://hdl.handle.net/10261/11906
Título

RNA is an integral component of chromatin that contributes to its structural organization

AutorRodríguez-Campos, Antonio; Azorín, Ferran
Fecha de publicación14-nov-2007
EditorPublic Library of Science
CitaciónPLoS ONE 2(11): e1182 (2007)
ResumenChromatin structure is influenced by multiples factors, such as pH, temperature, nature and concentration of counterions, post-translational modifications of histones and binding of structural non-histone proteins. RNA is also known to contribute to the regulation of chromatin structure as chromatin-induced gene silencing was shown to depend on the RNAi machinery in S. pombe, plants and Drosophila. Moreover, both in Drosophila and mammals, dosage compensation requires the contribution of specific non-coding RNAs. However, whether RNA itself plays a direct structural role in chromatin is not known. Here, we report results that indicate a general structural role for RNA in eukaryotic chromatin. RNA is found associated to purified chromatin prepared from chicken liver, or cultured Drosophila S2 cells, and treatment with RNase A alters the structural properties of chromatin. Our results indicate that chromatin-associated RNAs, which account for 2%–5% of total chromatin-associated nucleic acids, are polyA− and show a size similar to that of the DNA contained in the corresponding chromatin fragments. Chromatin-associated RNA(s) are not likely to correspond to nascent transcripts as they are also found bound to chromatin when cells are treated with α-amanitin. After treatment with RNase A, chromatin fragments of molecular weight >3.000 bp of DNA showed reduced sedimentation through sucrose gradients and increased sensitivity to micrococcal nuclease digestion. This structural transition, which is observed both at euchromatic and heterochromatic regions, proceeds without loss of histone H1 or any significant change in core-histone composition and integrity.
Descripción12 pages, 8 figures.-- PMID: 18000552 [PubMed].-- PMCID: PMC2063516.
Versión del editorhttp://dx.doi.org/10.1371/journal.pone.0001182
URIhttp://hdl.handle.net/10261/11906
DOI10.1371/journal.pone.0001182
ISSN1932-6203
Aparece en las colecciones: (IBMB) Artículos
Ficheros en este ítem:
Fichero Descripción Tamaño Formato  
Rodriguez_Azorin_PLoS_ONE_2_11_2007.pdf3,02 MBAdobe PDFVista previa
Visualizar/Abrir
Mostrar el registro completo
 

Artículos relacionados:


NOTA: Los ítems de Digital.CSIC están protegidos por copyright, con todos los derechos reservados, a menos que se indique lo contrario.