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Application of ring study: Water toxicity determinations by bioluminescence assay with Vibrio fischeri

AuthorsHernando, María Dolores; Malato, O.; Farré Urgell, Marinel.la; Fernández Alba, Amadeo R.; Barceló, Damià
KeywordsVibrio fischeri
Water analysis
Issue Date15-Apr-2006
CitationTalanta 69(2): 370-376 (2006)
AbstractAcceptance of toxicity bioassays as effective analytical tools in environmental areas needs guarantees of standardization but also validation. Ten European laboratories took part in an inter-laboratory study using different commercial devices based on bioluminescence inhibition of bacteria Vibrio fischeri. Reproducibility and stability by short toxicity endpoints, effective concentration that gives 10%, 50% and 80% of inhibition (EC-10, EC-50 and EC-80) is evaluated. Parametric and non-parametric statistic is applied and performance of participant laboratories is addressed by z-scores calculated by non-parametric statistic. z-Score classification was based on harmonised protocol for proficiency testing of analytical laboratories (satisfactory |z| ≤ 2; questionable 2 < |z| ≤ 3; unsatisfactory |z| > 3). Tested samples were phenol, 3,5-dichlorophenol and influent wastewater. Based on z-score classification, more than 70% of the laboratories showed a satisfactory performance for phenol, 3,5-dichlorophenol and influent wastewater (86%, 90% and 70%, respectively). Reproducibility and stability was observed in toxicant references and in wastewater samples. EC-80 determination appears to be more robust that EC-10 and EC-50. EC determinations can be considered favorable at 5 and 15 min of exposition, in particular for EC-80. The use of different commercial devices can not be considered an additional source of variation.
Description7 pages, 5 figures.-- PMID: 18970576 [PubMed].-- Available online Nov 21, 2005.-- Issue title: "1st Swift-WFD workshop on validation of Robustness of sensors and bioassays for Screening Pollutants" (Maó, Menorca, Dec 2-3, 2004).
Publisher version (URL)http://dx.doi.org/10.1016/j.talanta.2005.09.039
Appears in Collections:(IDAEA) Artículos
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