English   español  
Por favor, use este identificador para citar o enlazar a este item: http://hdl.handle.net/10261/11884
COMPARTIR / IMPACTO:
Estadísticas
logo share SHARE logo core CORE   Add this article to your Mendeley library MendeleyBASE

Visualizar otros formatos: MARC | Dublin Core | RDF | ORE | MODS | METS | DIDL
Exportar a otros formatos:
Título

Kinetic Spectroscopy of Erythrosin Phosphorescence and Delayed Fluorescence in Aqueous Solution at Room Temperature

AutorDuchowicz, Ricardo; Ferrer, M. Luisa ; Acuña, A. Ulises
Palabras claveKinetic Spectroscopy
Erythrosin Phosphorescence
Fecha de publicaciónmay-1998
EditorAmerican Society for Photobiology
CitaciónPhotochemistry and Photobiology, 68, 494-501 (1998)
ResumenThe photophysics and polarization of the phosphorescence and delayed fluorescence of erythrosin in conditions compatible with the current biological applications of the dye (aqueous buffers at pH 7.4 at ambient temperatures) and in ethanol have been studied as a function of dye concentration (10 −7-10−5M) and temperature (245–333K). The emission decay is strictly single exponential and the detailed kinetic analysis of all the rate processes connected with the emitting T1 state showed that (1) the lowering of the emission lifetime at the higher temperatures is due to a very efficient self-quenching process, (2) the back intersystem crossing rate Tx S1 is temperature dependent (δETS7 kcal mol−1) but the T1S0 is not (Ea0.1 kcal mol−1) and (3) both intersystem crossing processes are very sensitive to solvent polarity, which accounts for the solvent dependence of the phosphorescence yield and lifetime. The high value of the phosphorescence anisotropy (r0= 0.25 lt 0.006) is independent of the excitation and emission wavelengths, and its evolution in time accurately reflects the rotational restrictions in solid solutions. The relevance of these findings to studies with protein-dye conjugates is also outlined to facilitate the design and interpretation of phosphorescence depolarization experiments that probe the (μs-ms dynamics of biomolecules and supramolecular systems.
Versión del editorhttp://dx.doi.org/10.1111/j.1751-1097.1998.tb02505.x
URIhttp://hdl.handle.net/10261/11884
DOI10.1111/j.1751-1097.1998.tb02505.x
ISSN1751-1097 (Online)
0031-8655 (Print)
Aparece en las colecciones: (IQFR) Artículos
Ficheros en este ítem:
No hay ficheros asociados a este ítem.
Mostrar el registro completo
 


NOTA: Los ítems de Digital.CSIC están protegidos por copyright, con todos los derechos reservados, a menos que se indique lo contrario.