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Functional characterization of E- and P-cadherin in invasive breast cancer cells
|Authors:||Sarrió, David ; Palacios Calvo, José ; Hergueta-Redondo, Marta; Gómez-López, Gonzalo; Cano, Amparo ; Moreno-Bueno, Gema|
|Keywords:||Invasive breast cancer|
Cadherin-catenin adhesion complexes
|Citation:||BMC Cancer 9: 74 (2009)|
|Abstract:||[Background]: Alterations in the cadherin-catenin adhesion complexes are involved in tumor initiation, progression and metastasis. However, the functional implication of distinct cadherin types in breast cancer biology is still poorly understood.|
[Methods]: To compare the functional role of E-cadherin and P-cadherin in invasive breast cancer, we stably transfected these molecules into the MDA-MB-231 cell line, and investigated their effects on motility, invasion and gene expression regulation.
[Results]: Expression of either E- and P-cadherin significantly increased cell aggregation and induced a switch from fibroblastic to epithelial morphology. Although expression of these cadherins did not completely reverse the mesenchymal phenotype of MDA-MB-231 cells, both E- and P-cadherin decreased fibroblast-like migration and invasion through extracellular matrix in a similar way. Moreover, microarray gene expression analysis of MDA-MB-231 cells after expression of E- and P-cadherins revealed that these molecules can activate signaling pathways leading to significant changes in gene expression. Although the expression patterns induced by E- and P-cadherin showed more similarities than differences, 40 genes were differentially modified by the expression of either cadherin type.
[Conclusion]: E- and P-cadherin have similar functional consequences on the phenotype and invasive behavior of MDA-MB-231 cells. Moreover, we demonstrate for the first time that these cadherins can induce both common and specific gene expression programs on invasive breast cancer cells. Importantly, these identified genes are potential targets for future studies on the functional consequences of altered cadherin expression in human breast cancer.
|Description:||14 páginas, 4 figuras.-- PMID: 19257890 [PubMed].-- PMCID: PMC2656544.-- Información adicional (Suppl. files S1-S6) disponible en: http://www.biomedcentral.com/1471-2407/9/74/additional/|
Additional files: 1. Wound healing assay 231-control cells.-- 2. Wound healing assay 231-E-cadherin cells.-- 3. Wound healing assay 231-P-cadherin cells.-- 4. Complete list of genes modulated by E-cadherin and/or P-cadherin (at least 2 fold with respect to control cells) in 231 cells.-- 5. Potential signaling networks mediated by E-cadherin-regulated genes at the biological process level.-- 6. Potential signaling networks mediated by P-cadherin-regulated genes at the biological process level.
|Publisher version (URL):||http://dx.doi.org/10.1186/1471-2407-9-74|
|Appears in Collections:||(IIBM) Artículos|
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|Sarrio_et_al_BMC_Cancer_9_2009.pdf||Main text file||6,61 MB||Adobe PDF|
|1471-2407-9-74-s1.mov||Suppl. file 1 (MOV format)||7,27 MB||Video Quicktime||View/Open|
|1471-2407-9-74-s2.mov||Suppl. file 2 (MOV format)||8,15 MB||Video Quicktime||View/Open|
|1471-2407-9-74-s3.mov||Suppl. file 3 (MOV format)||3,86 MB||Video Quicktime||View/Open|
|1471-2407-9-74-s4.doc||Suppl. file 4||306,5 kB||Microsoft Word||View/Open|
|1471-2407-9-74-s5.pdf||Suppl. file 5||1,38 MB||Adobe PDF|
|1471-2407-9-74-s6.pdf||Suppl. file 6||438,11 kB||Adobe PDF|