Por favor, use este identificador para citar o enlazar a este item: http://hdl.handle.net/10261/118199
COMPARTIR / EXPORTAR:
logo share SHARE logo core CORE BASE
Visualizar otros formatos: MARC | Dublin Core | RDF | ORE | MODS | METS | DIDL | DATACITE

Invitar a revisión por pares abierta
Campo DC Valor Lengua/Idioma
dc.contributor.authorSolís, María Teresaes_ES
dc.contributor.authorEl-Tantawy, Ahmed-Abdallaes_ES
dc.contributor.authorCano, Vanesaes_ES
dc.contributor.authorRisueño, María Carmenes_ES
dc.contributor.authorTestillano, Pilar S.es_ES
dc.date.accessioned2015-07-21T09:29:53Z-
dc.date.available2015-07-21T09:29:53Z-
dc.date.issued2015-06-25-
dc.identifier.citationFrontiers in Plant Science 6: 472 (2015)es_ES
dc.identifier.issn1664-462X-
dc.identifier.urihttp://hdl.handle.net/10261/118199-
dc.description17 p.-10 fig.es_ES
dc.description.abstractMicrospores are reprogrammed by stress in vitro toward embryogenesis. This process is an important tool in breeding to obtain double-haploid plants. DNA methylation is a major epigenetic modification that changes in differentiation and proliferation. We have shown changes in global DNA methylation during microspore reprogramming. 5-Azacytidine (AzaC) cannot be methylated and leads to DNA hypomethylation. AzaC is a useful demethylating agent to study DNA dynamics, with a potential application in microspore embryogenesis. This work analyzes the effects of short and long AzaC treatments on microspore embryogenesis initiation and progression in two species, the dicot Brassica napus and the monocot Hordeum vulgare. This involved the quantitative analyses of proembryo and embryo production, the quantification of DNA methylation, 5-methyl-deoxy-cytidine (5mdC) immunofluorescence and confocal microscopy, and the analysis of chromatin organization (condensation/decondensation) by light and electron microscopy. Four days of AzaC treatments (2.5 μM) increased embryo induction, response associated with a decrease of DNA methylation, modified 5mdC, and heterochromatin patterns compared to untreated embryos. By contrast, longer AzaC treatments diminished embryo production. Similar effects were found in both species, indicating that DNA demethylation promotes microspore reprogramming, totipotency acquisition, and embryogenesis initiation, while embryo differentiation requires de novo DNA methylation and is prevented by AzaC. This suggests a role for DNA methylation in the repression of microspore reprogramming and possibly totipotency acquisition. Results provide new insights into the role of epigenetic modifications in microspore embryogenesis and suggest a potential benefit of inhibitors, such as AzaC, to improve the process efficiency in biotechnology and breeding programs.es_ES
dc.description.sponsorshipWork supported by projects (references BFU2008-00203, BFU2011-23752, AGL2014-52028-R) funded by the Spanish Ministry of Economy and Competitiveness (MINECO) and the European Regional Development Fund (ERDF/FEDER). AAET is recipient of a predoctoral fellowship of the JAE-Pre Program of the Spanish National Research Council, CSIC (JAEPre2010-052), cofunded by ERDF/FEDER.es_ES
dc.language.isoenges_ES
dc.publisherFrontiers Mediaes_ES
dc.relation.isversionofPublisher's versiones_ES
dc.rightsopenAccesses_ES
dc.subjectMicrospore culturees_ES
dc.subjectEpigenetic inhibitorses_ES
dc.subjectDemethylating agentses_ES
dc.subjectTotipotencyes_ES
dc.subjectMicrospore reprogramminges_ES
dc.subjectHordeum vulgarees_ES
dc.subjectBrassica napuses_ES
dc.title5-azacytidine promotes microspore embryogenesis initiation by decreasing global DNA methylation,but prevents subsequent embryo development in rapeseed and barleyes_ES
dc.typeartículoes_ES
dc.identifier.doi10.3389/fpls.2015.00472-
dc.description.peerreviewedPeer reviewedes_ES
dc.relation.publisherversionhttp://dx.doi.org/ 10.3389/fpls.2015.00472es_ES
dc.identifier.e-issn1664-462X,-
dc.rights.licensehttp://creativecommons.org/licenses/by/4.0/es_ES
dc.relation.csices_ES
dc.identifier.pmid26161085-
dc.type.coarhttp://purl.org/coar/resource_type/c_6501es_ES
item.openairetypeartículo-
item.cerifentitytypePublications-
item.languageiso639-1en-
item.grantfulltextopen-
item.openairecristypehttp://purl.org/coar/resource_type/c_18cf-
item.fulltextWith Fulltext-
Aparece en las colecciones: (CIB) Artículos
Ficheros en este ítem:
Fichero Descripción Tamaño Formato
Solis et al 2015 FrontiersPS.pdf4,55 MBAdobe PDFVista previa
Visualizar/Abrir
Show simple item record

CORE Recommender

PubMed Central
Citations

32
checked on 16-mar-2024

SCOPUSTM   
Citations

71
checked on 23-mar-2024

WEB OF SCIENCETM
Citations

65
checked on 27-feb-2024

Page view(s)

379
checked on 29-mar-2024

Download(s)

316
checked on 29-mar-2024

Google ScholarTM

Check

Altmetric

Altmetric


Artículos relacionados:


Este item está licenciado bajo una Licencia Creative Commons Creative Commons