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dc.contributor.authorVillagrasa, Marta-
dc.contributor.authorGuillamón, Miriam-
dc.contributor.authorEljarrat, Ethel-
dc.contributor.authorBarceló, Damià-
dc.identifier.citationJournal of Agriculture and Food Chemistry 54 (4):1001-1008 (2006)en_US
dc.description8 pages, 6 tables, 4 figures.en_US
dc.description.abstractA new analytical method based on the use of pressurized liquid extraction (PLE) followed by solid-phase extraction with LiChrolut RP C18 cartridges was evaluated for the sample preparation, extraction, and cleanup of eight naturally occurring benzoxazinone derivatives, 2-β-d-glucopyranosyloxy-4-hydroxy-1,4-benzoxazin-3-one, 2-β-d-glucopyranosyloxy-4-hydroxy-7-methoxy-1,4-benzoxazin-3-one, 2,4-dihydroxy-1,4-benzoxazin-3-one (DIBOA), 2,4-dihydroxy-7-methoxy-1,4-benzoxazin-3-one, 2-hydroxy-1,4-benzoxazin-3-one, 2-hydroxy-7-methoxy-1,4-benzoxazin-3-one, benzoxazolin-2-one, and 6-methoxybenzoxazolin-2-one in plant samples. Afterward, liquid chromatography−electrospray mass spectrometry, using the selected ion monitoring mode and internal standard (2-MeO-DIBOA, indoxyl-β-d-glucoside, and quercetin-3-O-rutinoside) quantification method was performed. This paper demonstrates the effectiveness of the PLE method, in conjunction with sensitive and specific mass spectrometric detection, for the quantitative recovery of compounds of the benzoxazinone class from plants. The recoveries of the analytes ranged from 66 to 110% with coefficients of variation ranging from 1 to 14%. This method gave detection limits between 1 and 27 μg/g. The method was applied to foliage and roots of three different wheat cultivars, and the analytes were detected in the range of 11−3261 μg/g of dry weight.en_US
dc.description.sponsorshipWe thank Prof. D. Sicker (University of Leipzig), Prof. S. Chilton (University of North Carolina), Dr. F. Macías (University of Cadiz), Prof. Dr. Hajime Iwamura (Kyoto University), and Prof. Dr. Lisbeth Jonsson (Södertorn University College) for kindly providing standards, and Dr. V. Cambier (Universite´ Catholique de Louvain) and Dr. C. Werner (University of Zurich) for helpful discussions. We greatly appeciate the generous gift of the Phenomenex Synergi MAX-RP HPLC column from Daniel Lopez (JASCO Analytica, Spain) and technical assistance provided by D. Fanjul and R. Chaler (IIQAB-CSIC). Finally, we thank Merck for supplying the SPE cartridgesen_US
dc.format.extent19968 bytes-
dc.publisherAmerican Chemical Societyen_US
dc.subjectBenzoxazinone derivativesen_US
dc.subjectPressurized liquid extractionen_US
dc.subjectLiquid chromatographyen_US
dc.subjectMass spectrometry detectionen_US
dc.titleDetermination of Benzoxazinone Derivatives in Plants by Combining Chromatography−Electrospray Mass Spectrometryen_US
dc.description.peerreviewedPeer revieweden_US
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