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Determination of Benzoxazinone Derivatives in Plants by Combining Chromatography−Electrospray Mass Spectrometry

AuthorsVillagrasa, Marta; Guillamón, Miriam; Eljarrat, Ethel ; Barceló, Damià
KeywordsBenzoxazinone derivatives
Pressurized liquid extraction
Liquid chromatography
Mass spectrometry detection
Issue Date24-Jan-2006
PublisherAmerican Chemical Society
CitationJournal of Agriculture and Food Chemistry 54 (4):1001-1008 (2006)
AbstractA new analytical method based on the use of pressurized liquid extraction (PLE) followed by solid-phase extraction with LiChrolut RP C18 cartridges was evaluated for the sample preparation, extraction, and cleanup of eight naturally occurring benzoxazinone derivatives, 2-β-d-glucopyranosyloxy-4-hydroxy-1,4-benzoxazin-3-one, 2-β-d-glucopyranosyloxy-4-hydroxy-7-methoxy-1,4-benzoxazin-3-one, 2,4-dihydroxy-1,4-benzoxazin-3-one (DIBOA), 2,4-dihydroxy-7-methoxy-1,4-benzoxazin-3-one, 2-hydroxy-1,4-benzoxazin-3-one, 2-hydroxy-7-methoxy-1,4-benzoxazin-3-one, benzoxazolin-2-one, and 6-methoxybenzoxazolin-2-one in plant samples. Afterward, liquid chromatography−electrospray mass spectrometry, using the selected ion monitoring mode and internal standard (2-MeO-DIBOA, indoxyl-β-d-glucoside, and quercetin-3-O-rutinoside) quantification method was performed. This paper demonstrates the effectiveness of the PLE method, in conjunction with sensitive and specific mass spectrometric detection, for the quantitative recovery of compounds of the benzoxazinone class from plants. The recoveries of the analytes ranged from 66 to 110% with coefficients of variation ranging from 1 to 14%. This method gave detection limits between 1 and 27 μg/g. The method was applied to foliage and roots of three different wheat cultivars, and the analytes were detected in the range of 11−3261 μg/g of dry weight.
Description8 pages, 6 tables, 4 figures.
Publisher version (URL)http://dx.doi.org/10.1021/jf050897p
Appears in Collections:(IDAEA) Artículos
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