Por favor, use este identificador para citar o enlazar a este item:
http://hdl.handle.net/10261/118075
COMPARTIR / EXPORTAR:
SHARE CORE BASE | |
Visualizar otros formatos: MARC | Dublin Core | RDF | ORE | MODS | METS | DIDL | DATACITE | |
Título: | Topoisomerase II minimizes DNA entanglements by proofreading DNA topology after DNA strand passage |
Autor: | Martínez-García, Belén CSIC ORCID ; Fernàndez, Xavier CSIC; Díaz-Ingelmo, Ofelia CSIC ORCID ; Rodríguez-Campos, Antonio CSIC; Manichanh, Chaysavanh CSIC ORCID; Roca, Joaquim CSIC ORCID | Fecha de publicación: | 31-oct-2013 | Editor: | Oxford University Press | Citación: | Nucleic Acids Research 42: 1821- 1830 (2014) | Resumen: | By transporting one DNA double helix (T-segment) through a double-strand break in another (G-segment), topoisomerase II reduces fractions of DNA catenanes, knots and supercoils to below equilibrium values. How DNA segments are selected to simplify the equilibrium DNA topology is enigmatic, and the biological relevance of this activity is unclear. Here we examined the transit of the T-segment across the three gates of topoisomerase II (entry N-gate, DNA-gate and exit C-gate). Our experimental results uncovered that DNA transport probability is determined not only during the capture of a T-segment at the N-gate. When a captured T-segment has crossed the DNA-gate, it can backtrack to the N-gate instead of exiting by the C-gate. When such backtracking is precluded by locking the N-gate or by removing the C-gate, topoisomerase II no longer simplifies equilibrium DNA topology. Therefore, we conclude that the C-gate enables a post-DNA passage proofreading mechanism, which challenges the release of passed T-segments to either complete or cancel DNA transport. This proofreading activity not only clarifies how type-IIA topoisomerases simplify the equilibrium topology of DNA in free solution, but it may explain also why these enzymes are able to solve the topological constraints of intracellular DNA without randomly entangling adjacent chromosomal regions. | Versión del editor: | http://dx.doi.org/10.1093/nar/gkt1037 | URI: | http://hdl.handle.net/10261/118075 | DOI: | 10.1093/nar/gkt1037 | Identificadores: | doi: 10.1093/nar/gkt1037 issn: 1362-4962 |
Aparece en las colecciones: | (IBMB) Artículos |
Ficheros en este ítem:
Fichero | Descripción | Tamaño | Formato | |
---|---|---|---|---|
Martinez-Garcia-Nuc-Acids-Res-2014-v42-n3-p1821.pdf | 6,23 MB | Unknown | Visualizar/Abrir |
CORE Recommender
PubMed Central
Citations
11
checked on 06-abr-2024
SCOPUSTM
Citations
21
checked on 16-abr-2024
WEB OF SCIENCETM
Citations
19
checked on 25-feb-2024
Page view(s)
398
checked on 19-abr-2024
Download(s)
235
checked on 19-abr-2024
Google ScholarTM
Check
Altmetric
Altmetric
Artículos relacionados:
NOTA: Los ítems de Digital.CSIC están protegidos por copyright, con todos los derechos reservados, a menos que se indique lo contrario.