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Formation of ABTS radical cation in the presence of silver nanoparticles as revealed by surface-enhanced Raman scattering

AuthorsGarcía-Leis, Adianez ; García-Ramos, José Vicente ; Jurasekova, Z. ; Fabriciova, G. ; Antalik, Marian; Jancura, Daniel; Sánchez-Cortés, Santiago
Issue Date9-Jul-2014
CitationXXIV Reunión Nacional de Espectroscopia- VIII Congreso Ibérico de Espectroscopía (2014)
AbstractABTS [2,2¿-azinobis(3-ethylbenzothiazoline-6-sulfonic acid)] has been extensively employed to evaluate the free radical trapping capacity of pure compounds and complex mixtures such as biological fluids or foods [1], when it is incubated in presence of a free radical source in the so-called Trolox equivalent antioxidant capacity (TEAC) assay. The latter assay is performed by using a colorimetric experiment, where ABTS is previously oxidized by persulfate to get the radical cation ABTS*+ [2], giving rise to an intense absorbance at 734 nm. In this work we report the formation of the radical cation in the presence of star-shaped silver nanoparticles (AgNS) [3]. ABTS*+ was characterized by surface-enhanced Raman scattering (SERS) and detected at trace concentrations (2 µM). The ABTS*+/AgNS system is proposed here as a reliable substrate to test the antioxidant activity of foods based on the high sensitivity of plasmonic nanostars as an alternative method to the TEAC test
DescriptionEspectroscoía, Universidad de la Rioja, Logroño, La Rioja, 9-11 de julio de 2014. PO83; http://www.unirioja.es/espectroscopia2014/
Appears in Collections:(CFMAC-IEM) Comunicaciones congresos
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