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http://hdl.handle.net/10261/11750
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Campo DC | Valor | Lengua/Idioma |
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dc.contributor.author | Mauriz, Elba | - |
dc.contributor.author | Calle Martín, Ana | - |
dc.contributor.author | Abad, Antonio | - |
dc.contributor.author | Montoya, Ángel | - |
dc.contributor.author | Hildebrandt, Alain | - |
dc.contributor.author | Barceló, Damià | - |
dc.contributor.author | Lechuga, Laura M. | - |
dc.date.accessioned | 2009-03-19T13:19:24Z | - |
dc.date.available | 2009-03-19T13:19:24Z | - |
dc.date.issued | 2006-05-15 | - |
dc.identifier.citation | Biosensors & Bioelectronics 21(11): 2129-2336 (2006) | en_US |
dc.identifier.issn | 0956-5663 | - |
dc.identifier.uri | http://hdl.handle.net/10261/11750 | - |
dc.description | 8 pages, 3 tables, 5 figures.-- PMID: 16309901 [PubMed].-- Available online Nov 23, 2005. | en_US |
dc.description.abstract | The analysis of carbaryl in natural water samples was accomplished using a portable immunosensor based on surface plasmon resonance (SPR) technology. The assay was based on a binding inhibition immunoassay format with the analyte derivative covalently immobilized on the sensor surface. An alkanethiol self-assembled monolayer (SAM) was formed onto the gold-coated sensor surface to allow the reusability of the same sensing surface during 220 regeneration cycles. Reproducibility was evaluated by performing three independent assays in triplicate on 3 different days. The batch-assay variability was also calculated using three different gold-coated sensor surfaces. The intra- and inter-day relative standard deviation were 8.6 and 15.3%, respectively, whilst a variation of 7.4% in assay sensitivity was obtained by employing different sensor chips. The lowest detection limit, calculated as the concentration providing a 10% decrease of the blank signal, was of 1.38 μg L−1. Matrix effects were also evaluated in different water types, showing I50 values (carbaryl concentrations that produced a 50% decrease of the blank signal) within the range of carbaryl standard curves in distilled water (2.78–3.55 μg L−1). The carbaryl immunoassay performance was validated with respect to conventional high-performance liquid chromatography–mass spectrometry (HPLC–MS). The correlation between methods was in good agreement (r2 = 0.998, 0.999 and 0.999) for the three types of natural water samples tested. A complete assay cycle, including regeneration, is accomplished in 20 min. All measurements were carried out with the SPR sensor system (β-SPR) commercialised by the company SENSIA, SL (Spain). The small size and low-time of response of the β-SPR platform would allow its utilization in real contaminated locations. | en_US |
dc.description.sponsorship | This work has been supported by CSIC project, DESREMOL (2004 20FO280). | en_US |
dc.format.extent | 162 bytes | - |
dc.format.mimetype | application/msword | - |
dc.language.iso | eng | en_US |
dc.publisher | Elsevier | en_US |
dc.rights | closedAccess | en_US |
dc.subject | Surface plasmon resonance (SPR) | en_US |
dc.subject | Immunosensor | en_US |
dc.subject | Self-assembled monolayer | en_US |
dc.subject | Carbaryl | en_US |
dc.subject | Validation | en_US |
dc.subject | Environmental analysis | en_US |
dc.title | Determination of carbaryl in natural water samples by a surface plasmon resonance flow-through immunosensor | en_US |
dc.type | artículo | en_US |
dc.identifier.doi | 10.1016/j.bios.2005.10.013 | - |
dc.description.peerreviewed | Peer reviewed | en_US |
dc.relation.publisherversion | http://dx.doi.org/10.1016/j.bios.2005.10.013 | en_US |
dc.type.coar | http://purl.org/coar/resource_type/c_6501 | es_ES |
item.openairetype | artículo | - |
item.grantfulltext | none | - |
item.cerifentitytype | Publications | - |
item.openairecristype | http://purl.org/coar/resource_type/c_18cf | - |
item.fulltext | No Fulltext | - |
item.languageiso639-1 | en | - |
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