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Intestinal IL-6 controls immune inflammation in human ulcerative colitis and induces a pro-inflammatory skin homing phenotype in dendritic cells and T-cells they stimulate

AuthorsBernardo, David; Vallejo-Díez, Sara; Mann, Elizabeth R.; Al-Hassi, Hafid O.; Martínez-Abad, Beatriz; Montalvillo, Enrique; Fernández-Salazar, Luis; Garrote, José Antonio ; Arranz, Eduardo ; Knight, Stella C.
Issue Date2012
CitationEMIG 2012
Abstract[Background]: Dendritic cells (DC) control the type and site of immune responses. Ulcerative colitis (UC) is considered a TH2 disease mediated by IL-13 where up to 1/3 of the patients can develop extra-intestinal manifestations. We hypothesised that local homeostatic factors are masked by ongoing inflammation in inflamed areas of the gut. [Methods]: Colonic biopsies from inflamed and non-inflamed areas of UC patients were cultured in vitro. Cytokine secretion in culture supernatants was determined. Cell-free supernatants were used to condition human blood enriched DC from healthy volunteers. Phenotype and function of DC was determined by flow cytometry and mixed leukocyte reactions respectively. [Results]: Levels of IL-13 in the culture supernatants were below the detection limit in most cases and the cytokine profile suggested a mixed profile rather than a TH2 cytokine profile. IL-6 was the predominant cytokine found in inflamed areas from UC patients and its concentration correlated with the Mayo endoscopic score for severity of disease. DC conditioned with non-inflamed areas from UC patients acquired a regulatory phenotype with decreased stimulatory capacity. However, DC conditioned with inflamed areas acquired a pro-inflammatory phenotype, increased expression of skin homing CCR8, did not decrease their stimulatory capacity for T-cells and primed them with the skin-homing CLA molecule in an IL-6 dependent mechanism. [Conclusion]: Our results highlight the role of IL-6 in UC, question the concept of UC as a TH2 disease, the relevance of IL-13 in its aetiology and provide a molecular explanation for the development of extra-intestinal manifestations in IBD patients.
DescriptionResumen del póster presentado al 8th European Mucosal Immunology Meeting celebrado en Dublín (Irlanda) del 10 al 13 de octubre de 2012.-- et al.
Appears in Collections:(IBGM) Comunicaciones congresos
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