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Título

DC-SIGN/HLA-DR+ cells increased in coeliac duodenum

AutorMontalvillo, Enrique; García-Vallejo, Juan Jesús; Martínez-Abad, Beatriz; Vallejo-Díez, Sara; Escudero-Hernández, Celia; Garrote, José Antonio ; Arranz, Eduardo
Fecha de publicación2012
CitaciónEMIG 2012
ResumenWe aimed to determine the DC-SIGN expression by immunofluorescence in duodenal biopsies from coeliac patients and controls. We obtained endoscopic duodenal biopsies from 3 active Coeliac Disease (aCD), 3 Gluten Free Diet (GFD) and 3 Healthy Controls (HC). The samples were frozen in liquid nitrogen. After cutting 8μm tissue sections, slides were dried overnight. The staining was done adding rabbit DC-Sign anti-human, labelled with α-rabbit AlexaFluor 488 and mouse HLA-DR anti-human or mouse CD4 anti-human, labelled with α-mouse AlexaFluor 647 for 1h at room temperature. We used the corresponding isotypes as controls. Slides were mounted using Vinol mounting medium with DAPIand viewed/analysed in a Leica DM6000 microscope. DC-SIGN was found to be most highly expressed in the lamina propria cells of aCD patients. In these patients, we have observed that all the DC-SIGN+ cells are HLA-DR+. On the contrary, both HC and GFD show less DC-SIGN/HLA-DR+ cells. Also these samples show some HLA-DR+ cells that do not express DC-SIGN. In aCD CD4+ T cell infiltration occurs in both the lamina propria and the epithelium. The T cells infiltration is accompanied with an increase in both the number of DC-SIGN+ cells and the expression level of DC-SIGN on HLA-DR+ cells in the lamina propria. This appears to be specific of aCD, as HC do not show CD4+ T cell infiltration and/or increased presence of DC-SIGN+. GFDpatients also show a lower CD4+ T cell infiltrate and DC-SIGN expression. This decreased depends on the time elapsed since the start of the gluten-free diet.
DescripciónPóster presentado al 8th European Mucosal Immunology Meeting celebrado en Dublín (Irlanda) del 10 al 13 de octubre de 2012.
URIhttp://hdl.handle.net/10261/117217
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