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Role of group IVA phospholipase A2 in adipocyte differentiation

AutorPeña, Lucía ; Balboa, María A.
Fecha de publicación2012
Citación4th European Workshop on Lipid Mediators (2012)
ResumenPhospholipases A2 (PLA2s) are enzymes that catalyze the hydrolysis of the sn-2 fatty acid of glycerophospholipids, generating a free fatty acid and a 2-lysophospholipid. In mammalians, more than 20 PLA2 forms have been identified to date. One of the better characterized is group IVA PLA2, also known as cytosolic PLA2alpha, an enzyme responsible for the release of the arachidonic acid that is used for prostaglandin synthesis. To define the contribution of this enzyme to adipocyte differentiation, we have performed in vitro experiments using the hormoneinduced 3T3-L1 cell model of adipocyte differentiation. Down-regulation of cPLA2alpha in 3T3-L1 preadipocytes using siRNA technology results in a decreased accumulation of neutral lipids in differentiated adipocytes, as measured by Oil Red O staining. Moreover, a reduction in the expression of key transcription factors during adipocyte differentiation process such as PPARgamma and C/EBPalpha, was also observed. As a consequence, expression of the adipocyte-related gen aP2/FABP4 had a low expression in cells treated with cPLA2alpha siRNA. Taken together these results suggest that cPLA2alpha is a key component of the regulation of transcriptional programs involved in the adipocyte differentiation in 3T3-L1 cells.
DescripciónResumen del póster presentado al Fourth European Workshop on Lipid Mediators celebrado en Paris (Francia) del 27 al 28 de septiembre de 2012.
URIhttp://hdl.handle.net/10261/117195
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