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dc.contributor.authorArsuaga, Javier-
dc.contributor.authorVázquez, Mariel-
dc.contributor.authorTrigueros, Sonia-
dc.contributor.authorSumners, De Witt-
dc.contributor.authorRoca, Joaquim-
dc.identifierdoi: 10.1073/pnas.032095099-
dc.identifierissn: 0027-8424-
dc.identifier.citationProceedings of the National Academy of Sciences of the United States of America 99(8): 5373-5377 (2002)-
dc.description.abstractWhen linear double-stranded DNA is packed inside bacteriophage capsids, it becomes highly compacted. However, the phage is believed to be fully effective only if the DNA is not entangled. Nevertheless, when DNA is extracted from a tailless mutant of the P4 phage, DNA is found to be cyclic and knotted (probability of 0.95). The knot spectrum is very complex, and most of the knots have a large number of crossings. We quantified the frequency and crossing numbers of these knots and concluded that, for the P4 tailless mutant, at least half the knotted molecules are formed while the DNA is still inside the viral capsid rather than during extraction. To analyze the origin of the knots formed inside the capsid, we compared our experimental results to Monte Carlo simulations of random knotting of equilateral polygons in confined volumes. These simulations showed that confinement of closed chains to tightly restricted volumes results in high knotting probabilities and the formation of knots with large crossing numbers. We conclude that the formation of the knots inside the viral capsid is driven mainly by the effects of confinement.-
dc.description.sponsorshipJ.A. and M.V. were supported by the Program in Mathematics and Molecular Biology through a Burroughs Wellcome Fund Interfaces Grant and by National Science Foundation Grant DMS-9971169. M.V. was supported by a Dirección General de Asuntos del Personal Académico-Universidad Nacional Autónoma de México graduate fellowship. This research was supported also by Ministry of Science of Spain Grant PB98-0487-
dc.publisherNational Academy of Sciences (U.S.)-
dc.titleKnotting probability of DNA molecules confined in restricted volumes: DNA knotting in phage capsids-
dc.description.versionPeer Reviewed-
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