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http://hdl.handle.net/10261/117020
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Campo DC | Valor | Lengua/Idioma |
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dc.contributor.author | Benavente, Rocio | - |
dc.contributor.author | Pessela, Benevides C. | - |
dc.contributor.author | Curiel, José Antonio | - |
dc.contributor.author | Rivas, Blanca de las | - |
dc.contributor.author | Muñoz, Rosario | - |
dc.contributor.author | Guisán, José Manuel | - |
dc.contributor.author | Mancheño, Jose M. | - |
dc.contributor.author | Cardelle-Cobas, Alejandra | - |
dc.contributor.author | Ruiz-Matute, Ana I. | - |
dc.contributor.author | Corzo, Nieves | - |
dc.date.accessioned | 2015-06-23T11:01:39Z | - |
dc.date.available | 2015-06-23T11:01:39Z | - |
dc.date.issued | 2015 | - |
dc.identifier | issn: 1420-3049 | - |
dc.identifier.citation | Molecules 20(5): 7874-7889 (2015) | - |
dc.identifier.uri | http://hdl.handle.net/10261/117020 | - |
dc.description | This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution license.-- This article belongs to the Section Natural Products. | - |
dc.description.abstract | A novel β-galactosidase from Lactobacillus plantarum (LPG) was over-expressed in E. coli and purified via a single chromatographic step by using lowly activated IMAC (immobilized metal for affinity chromatography) supports. The pure enzyme exhibited a high hydrolytic activity of 491 IU/mL towards o-nitrophenyl β-D-galactopyranoside. This value was conserved in the presence of different divalent cations and was quite resistant to the inhibition effects of different carbohydrates. The pure multimeric enzyme was stabilized by multipoint and multisubunit covalent attachment on glyoxyl-agarose. The glyoxyl-LPG immobilized preparation was over 20-fold more stable than the soluble enzyme or the one-point CNBr-LPG immobilized preparation at 50°C. This β-galactosidase was successfully used in the hydrolysis of lactose and lactulose and formation of different oligosaccharides was detected. High production of galacto-oligosaccharides (35%) and oligosaccharides derived from lactulose (30%) was found and, for the first time, a new oligosaccharide derived from lactulose, tentatively identified as 3′-galactosyl lactulose, has been described. | - |
dc.description.sponsorship | This work has been sponsored by Consolider INGENIO 2010 CSD2007-00063 FUNC-FOOD(CICYT), the Spanish Ministry of Science and Innovation (Project CTQ2009-07568), CSIC(Intramural Project 200980I133) and S2009/AGR-1469 (ALIBIRD) (CAM). | - |
dc.description.sponsorship | We acknowledge the support of the publication fee by the CSIC Open Access Publication Support Initiative through its Unit of Information Resources for Research (URICI). | - |
dc.publisher | Multidisciplinary Digital Publishing Institute | - |
dc.relation | S2009/AGR-1469/ALIBIRD | - |
dc.relation.isversionof | Publisher's version | - |
dc.rights | openAccess | - |
dc.subject | Lactose | - |
dc.subject | Lactulose | - |
dc.subject | Oligosaccharides synthesis | - |
dc.subject | Glyoxyl-agarose | - |
dc.subject | Immobilization | - |
dc.subject | β-galactosidase | - |
dc.subject | Lactobacillus plantarum | - |
dc.title | Improving properties of a novel β-galactosidase from Lactobacillus plantarum by covalent immobilization | - |
dc.type | artículo | - |
dc.identifier.doi | 10.3390/molecules20057874 | - |
dc.relation.publisherversion | http://dx.doi.org/10.3390/molecules20057874 | - |
dc.date.updated | 2015-06-23T11:01:39Z | - |
dc.description.version | Peer Reviewed | - |
dc.language.rfc3066 | eng | - |
dc.rights.license | http://creativecommons.org/licenses/by/4.0/ | - |
dc.contributor.funder | CSIC - Unidad de Recursos de Información Científica para la Investigación (URICI) | - |
dc.contributor.funder | Ministerio de Ciencia e Innovación (España) | - |
dc.contributor.funder | Consejo Superior de Investigaciones Científicas (España) | - |
dc.contributor.funder | Comisión Interministerial de Ciencia y Tecnología, CICYT (España) | - |
dc.contributor.funder | Comunidad de Madrid | - |
dc.relation.csic | Sí | - |
dc.identifier.funder | http://dx.doi.org/10.13039/501100004837 | es_ES |
dc.identifier.funder | http://dx.doi.org/10.13039/501100003339 | es_ES |
dc.identifier.funder | http://dx.doi.org/10.13039/501100007273 | es_ES |
dc.identifier.funder | http://dx.doi.org/10.13039/100012818 | es_ES |
dc.identifier.pmid | 25942370 | - |
dc.type.coar | http://purl.org/coar/resource_type/c_6501 | es_ES |
item.fulltext | With Fulltext | - |
item.openairecristype | http://purl.org/coar/resource_type/c_18cf | - |
item.cerifentitytype | Publications | - |
item.grantfulltext | open | - |
item.openairetype | artículo | - |
Aparece en las colecciones: | (ICTAN) Artículos (CIAL) Artículos (IQF) Artículos (ICP) Artículos |
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molecules-20-07874-v2.pdf | 1,39 MB | Adobe PDF | Visualizar/Abrir |
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