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Calcium imaging in neuron cell death

AuthorsCalvo-Rodríguez, María; Villalobos, Carlos ; Núñez, Lucía
KeywordsCalcium imagingimaging
Neuronneuron death
Issue Date2015
CitationNeuronal Cell Death: 73-85 (2015)
SeriesMethods in Molecular Biology
AbstractIntracellular Ca2+ is involved in control of a large variety of cell functions including apoptosis and neuron cell death . For example, intracellular Ca2+ overload is critical in neuron cell death induced by excitotoxicity . Thus, single cell monitoring of intracellular Ca2+ concentration ([Ca2+]cyt ) in neurons concurrently with apoptosis and neuron cell death is widely required. Procedures for culture and preparation of primary cultures of hippocampal rat neurons and fluorescence imaging of cytosolic Ca2+ concentration in Fura2/AM -loaded neurons are described. We also describe a method for apoptosis detection by immunofluorescence imaging . Finally, a simple method for concurrent measurements of [Ca2+]cyt and apoptosis in the same neurons is described.
Publisher version (URL)http://dx.doi.org/10.1007/978-1-4939-2152-2_6
Appears in Collections:(IBGM) Libros y partes de libros
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