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http://hdl.handle.net/10261/116987
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Campo DC | Valor | Lengua/Idioma |
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dc.contributor.author | Roca, Joaquim | es_ES |
dc.date.accessioned | 2015-06-22T11:54:42Z | - |
dc.date.available | 2015-06-22T11:54:42Z | - |
dc.date.issued | 2009-08-07 | - |
dc.identifier | doi: 10.1007/978-1-60761-340-4_3 | - |
dc.identifier | e-issn: 1940-6029 | - |
dc.identifier | issn: 1064-3745 | - |
dc.identifier.citation | Methods in molecular biology 582: 27-37 (2009) | es_ES |
dc.identifier.isbn | 978-1-60761-339-8 | - |
dc.identifier.isbn | 978-1-60761-340-4 | - |
dc.identifier.uri | http://hdl.handle.net/10261/116987 | - |
dc.description | Capítulo en: Duncan J. Clarke (ed.). DNA Topopisomerases : Methodes and Protocols. [S.l.]: Humana Press, 2009, p.23-37. (Methods in molecular biology ; 582, ISSN 1064-3745). ISBN 978-1-60761-339-8. ISBN 978-1-60761-340-4. DOI 10.1007/978-1-60761-340-4_3 | - |
dc.description.abstract | The electrophoretic velocity of a duplex DNA ring is mainly determined by its overall shape. Consequently, DNA topoisomers of opposite supercoiling handedness can have identical gel velocity, and topoisomers highly supercoiled cannot be separated beyond some point. These problems are overcome by two-dimensional agarose gel electrophoresis, which involves two successive electrophoresis steps in one gel slab. The first and second electrophoresis steps are conducted in orthogonal directions with different concentrations of DNA intercalating agents. These compounds alter the overall shape of the DNA and, thereby, change the relative mobility of individual DNA topoisomers. | - |
dc.publisher | Humana Press | es_ES |
dc.rights | closedAccess | - |
dc.subject | DNA supercoiling | - |
dc.subject | DNA linking number | - |
dc.subject | DNA twist | - |
dc.subject | DNA writhe | - |
dc.subject | Chloroquine | - |
dc.subject | Z-DNA | - |
dc.title | Two-dimensional agarose gel electrophoresis of DNA topoisomers | es_ES |
dc.type | capítulo de libro | es_ES |
dc.identifier.doi | 10.1007/978-1-60761-340-4_3 | - |
dc.relation.publisherversion | http://dx.doi.org/10.1007/978-1-60761-340-4_3 | - |
dc.date.updated | 2015-06-22T11:54:42Z | - |
dc.description.version | Peer Reviewed | - |
dc.language.rfc3066 | eng | - |
dc.relation.csic | Sí | es_ES |
dc.type.coar | http://purl.org/coar/resource_type/c_3248 | es_ES |
item.fulltext | No Fulltext | - |
item.openairecristype | http://purl.org/coar/resource_type/c_18cf | - |
item.cerifentitytype | Publications | - |
item.grantfulltext | none | - |
item.openairetype | capítulo de libro | - |
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