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Título

Asymmetric removal of supercoils suggests how topoisomerase II simplifies DNA topology

AutorTrigueros, Sonia; Salceda, Javier; Bermúdez, Ignacio; Fernàndez, Xavier; Roca, Joaquim
Palabras claveDNA binding
DNA topology
Supercoiled DNA
Topoisomerase
DNA bend
Fecha de publicación16-ene-2004
EditorAcademic Press
CitaciónJournal of Molecular Biology 335(3): 723-731 (2004)
ResumenType-IIA topoisomerases consume ATP as they catalyse the interconversion of DNA topoisomers by transporting one DNA segment through a transient break in another. It remains unclear how their activity simplifies the topology of DNA below equilibrium values. Here we report that eukaryotic topoisomerase II narrows the thermal distribution of DNA supercoils, by mainly removing negative DNA crossings. Surprisingly, this asymmetry in supercoil removal is not due to deformation of the DNA before strand passage. Topoisomerase II neither bends nor alters the helical conformation of the interacting DNA. Rather, it appears to interact with a third DNA segment, in addition to the gated and the transported segments. Remarkably, the simultaneous interaction with three DNA segments accounts for the asymmetric removal of supercoils in relaxed DNA and gives a clue to how topoisomerase II simplifies the topology of DNA against the thermal drive. © 2003 Elsevier Ltd. All rights reserved.
Versión del editorhttp://dx.doi.org/10.1016/j.jmb.2003.11.011
URIhttp://hdl.handle.net/10261/116971
DOI10.1016/j.jmb.2003.11.011
Identificadoresdoi: 10.1016/j.jmb.2003.11.011
issn: 0022-2836
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