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Título

An insight into the proteome of Crithidia fasciculata choanomastigotes as a comparative approach to axenic growth, peanut lectin agglutination and differentiation of Leishmania spp. promastigotes.

AutorAlcolea, Pedro J. ; Alonso, Ana ; García-Tabares, Francisco ; Toraño, Alfredo; Larraga, Vicente
Fecha de publicación11-dic-2014
EditorPublic Library of Science
CitaciónPLoS One. 2014 Dec 11;9(12):e113837
ResumenThe life cycle of the trypanosomatid Crithidia fasciculata is monogenetic, as the unique hosts of these parasites are different species of culicids. The comparison of these non-pathogenic microorganisms evolutionary close to other species of trypanosomatids that develop digenetic life cycles and cause chronic severe sickness to millions of people worldwide is of outstanding interest. A ground-breaking analysis of differential protein abundance in Crithidia fasciculata is reported herein. The comparison of the outcome with previous gene expression profiling studies developed in the related human pathogens of the genus Leishmania has revealed substantial differences between the motile stages of these closely related organisms in abundance of proteins involved in catabolism, redox homeostasis, intracellular signalling, and gene expression regulation. As L. major and L. infantum agglutinate with peanut lectin and non-agglutinating parasites are more infective, the agglutination properties were evaluated in C. fasciculata. The result is that choanomastigotes are able to agglutinate with peanut lectin and a non-agglutinating subpopulation can be also isolated. As a difference with L. infantum, the non-agglutinating subpopulation over-expresses the whole machinery for maintenance of redox homeostasis and the translation factors eIF5a, EF1α and EF2, what suggests a relationship between the lack of agglutination and a differentiation process.
Descripción26 p.-5 fig.-3 tab.
Versión del editorhttp://dx.doi.org/ 10.1371/journal.pone.0113837
URIhttp://hdl.handle.net/10261/116889
DOI10.1371/journal.pone.0113837
ISSN1932-6203
E-ISSN1932-6203
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