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Title

Progressive effects of N-myc deficiency on proliferation, neurogenesis, and morphogenesis in the olfactory epithelium

AuthorsWittmann, Walter; Schimmang, Thomas ; Gunhaga, Lena
KeywordsOlfactory epithelium
N-myc
Neurogenesis
Vomeronasal organ
Mouse
Issue Date2014
PublisherJohn Wiley & Sons
CitationDevelopmental Neurobiology 74(6): 643-656 (2014)
AbstractN-myc belongs to the myc proto-oncogene family, which is involved in numerous cellular processes such as proliferation, growth, apoptosis, and differentiation. Conditional deletion of N-myc in the mouse nervous system disrupted brain development, indicating that N-myc plays an essential role during neural development. How the development of the olfactory epithelium and neurogenesis within are affected by the loss of N-myc has, however, not been determined. To address these issues, we examined an N-mycFoxg1Cre conditional mouse line, in which N-myc is depleted in the olfactory epithelium. First changes in N-myc mutants were detected at E11.5, with reduced proliferation and neurogenesis in a slightly smaller olfactory epithelium. The phenotype was more pronounced at E13.5, with a complete lack of Hes5-positive progenitor cells, decreased proliferation, and neurogenesis. In addition, stereological analyses revealed reduced cell size of post-mitotic neurons in the olfactory epithelium, which contributed to a smaller olfactory pit. Furthermore, we observed diminished proliferation and neurogenesis also in the vomeronasal organ, which likewise was reduced in size. In addition, the generation of gonadotropin-releasing hormone neurons was severely reduced in N-myc mutants. Thus, diminished neurogenesis and proliferation in combination with smaller neurons might explain the morphological defects in the N-myc depleted olfactory structures. Moreover, our results suggest an important role for N-myc in regulating ongoing neurogenesis, in part by maintaining the Hes5-positive progenitor pool. In summary, our results provide evidence that N-myc deficiency in the olfactory epithelium progressively diminishes proliferation and neurogenesis with negative consequences at structural and cellular levels. © The Authors. Developmental Neurobiology Published by Wiley Periodicals, Inc.
DescriptionThis is an open access article under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License.
Publisher version (URL)http://dx.doi.org/10.1002/dneu.22162
URIhttp://hdl.handle.net/10261/116849
DOI10.1002/dneu.22162
Identifiersdoi: 10.1002/dneu.22162
issn: 1932-8451
e-issn: 1932-846X
Appears in Collections:(IBGM) Artículos
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