English   español  
Por favor, use este identificador para citar o enlazar a este item: http://hdl.handle.net/10261/116804
COMPARTIR / IMPACTO:
Estadísticas
logo share SHARE logo core CORE   Add this article to your Mendeley library MendeleyBASE

Visualizar otros formatos: MARC | Dublin Core | RDF | ORE | MODS | METS | DIDL
Exportar a otros formatos:
Título

MPP+ injection into rat substantia nigra causes secondary glial activation but not cell death in the ipsilateral striatum

AutorCanudas, Anna Maria; Friguls, Bibiana; Planas, Anna M. ; Gabriel, Cecília; Escubedo, Elena; Camarasa, Jorge; Camins, Antoni; Pallàs, Mercè
Palabras claveBinding
Immunohistochemistry
Rat
Reactive glia
[ 3H]PK 11195
Fecha de publicación2000
EditorAcademic Press
CitaciónNeurobiology of Disease 7(4): 343-361 (2000)
ResumenInjection of MPP+ into the substantia nigra causes extensive necrosis and anterograde degeneration of pars compacta dopaminergic neurons. We studied secondary effects in the ipsilateral striatum by examining dopaminergic terminals, signs of neuronal damage, and glial reactivity at 1, 2, 3, and 7 days after injection of MPP+ into the substantia nigra. Dopaminergic terminals and uptake sites were evaluated with [3H]GBR-12935 binding and tyrosine hydroxylase immunoreactivity. Glial reaction was examined with markers of astrocytes and microglia. Stereology was used to evaluate any changes in neuronal density. Tyrosine hydroxylase immunoreactivity and [3H]GBR-12935 binding markedly decreased (74%) from days 2 to 7. Loss of dopaminergic terminals in the ipsilateral striatum was accompanied by an intense astroglial and, to a lesser extent, microglial reaction. However, no signs of cell damage, neuronal loss, or disruption of the blood-brain barrier were found in the striatum. Resident astroglial and microglial cells showed a morphological shift and notable changes in protein expression typical of glial reactivity, yet the presence of macrophage-like cells was not detected. This study shows that injection of MPP+ in the substantia nigra causes a secondary reaction within the ipsilateral striatum involving the transformation of quiescent glia to reactive glia. It is suggested that stimuli derived from damaged dopaminergic terminals within the striatum are able to activate resident glia and that this glial transformation may promote repair and regeneration. (C) 2000 Academic Press.
Versión del editorhttp://dx.doi.org/10.1006/nbdi.2000.0308
URIhttp://hdl.handle.net/10261/116804
DOI10.1006/nbdi.2000.0308
Identificadoresdoi: 10.1006/nbdi.2000.0308
issn: 0969-9961
Aparece en las colecciones: (IIBB) Artículos
Ficheros en este ítem:
Fichero Descripción Tamaño Formato  
accesoRestringido.pdf15,38 kBAdobe PDFVista previa
Visualizar/Abrir
Mostrar el registro completo
 

Artículos relacionados:


NOTA: Los ítems de Digital.CSIC están protegidos por copyright, con todos los derechos reservados, a menos que se indique lo contrario.