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dc.contributor.authorVaquero, María Eugenia-
dc.contributor.authorEugenio, Laura I. de-
dc.contributor.authorMartínez, María Jesús-
dc.contributor.authorBarriuso, Jorge-
dc.date.issued2015-04-21-
dc.identifier.citationPLoS ONE 10(4): e0124882.es_ES
dc.identifier.issn1932-6203-
dc.identifier.urihttp://hdl.handle.net/10261/116781-
dc.description11 p.-4 fig.-1 tab.es_ES
dc.description.abstractThe fungus Pseudozyma antarctica produces a lipase (CalB) with broad substrate specificity, stability, high regio- and enantio-selectivity. It is active in non-aqueous organic solvents and at elevated temperatures. Hence, CalB is a robust biocatalyst for chemical conversions on an industrial scale. Here we report the in silico mining of public metagenomes and fungal genomes to discover novel lipases with high homology to CalB. The candidates were selected taking into account homology and conserved motifs criteria, as well as, phylogeny and 3D model analyses. The most promising candidate (PlicB) presented interesting structural properties. PlicB was expressed in a heterologous host, purified and partially characterized. Further experiments will allow finding novel catalytic properties with biotechnological interest.es_ES
dc.description.sponsorshipThis work was supported by the Spanish projects BIO2012-36372, RTC-2014-1777-3 and S2013/MAE-2907.es_ES
dc.language.isoenges_ES
dc.publisherPublic Library of Sciencees_ES
dc.rightsopenAccesses_ES
dc.titleA novel CalB-type lipase discovered by fungal genomes mininges_ES
dc.typeartículoes_ES
dc.identifier.doihttp://dx.doi.org/10.1371/journal.pone.0124882-
dc.description.peerreviewedPeer reviewedes_ES
dc.relation.publisherversionhttp://dx.doi.org/ 10.1371/journal.pone.0124882es_ES
dc.identifier.e-issn1932-6203-
dc.rights.licensehttp://creativecommons.org/licenses/by/4.0/es_ES
dc.relation.csices_ES
dc.identifier.pmid25898146-
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