English   español  
Por favor, use este identificador para citar o enlazar a este item: http://hdl.handle.net/10261/116780
logo share SHARE logo core CORE   Add this article to your Mendeley library MendeleyBASE

Visualizar otros formatos: MARC | Dublin Core | RDF | ORE | MODS | METS | DIDL
Exportar a otros formatos:

Inhibitors of NO-synthase and donors of NO modulate kainic acid-induced damage in the rat hippocampus

AutorGabriel, Cecília; Friguls, Bibiana; Sureda, Francesc Xavier; Pallàs, Mercè; Planas, Anna M. ; Escubedo, Elena; Camarasa, Jorge; Camins, Antoni
Palabras claveHeat shock proteins
[3H]PK 11195
Nitric oxide
Fecha de publicaciónene-2003
EditorJohn Wiley & Sons
CitaciónJournal of Neuroscience Research 59(6): 797-805 (2000)
ResumenThe effects of nitric oxide synthase (NOS) inhibitors, N(ω)-nitro-L-arginine and 7-nitroindazole, and the NOS substrate L-arginine on kainic acid (KA)-induced microglial reactivity and stress response were studied in the hippocampus 7 and 1 days after KA, respectively. Density of peripheral-type benzodiazepine receptors was measured as an index of microglial reactivity. Histological damage in hippocampus was evaluated at 7 days by neuronal counting. KA increased the maximal number of binding sites (B(max)) versus controls. Administration of either 7-nitroindazole (25 mg/kg) or N(ω)-nitro-L-arginine (20 and 50 mg/kg) 24 hr before KA, further increased B(max). This later effect was abolished by L-arginine (1 g/kg), which given 24 hr before KA decreased B(max) to control values. Also, KA-induced HSP72 stress response was attenuated by pre-treatment with L-arginine. Histological evaluation showed reduced cell numbers in the pyramidal cell layer of the hippocampus in groups receiving KA, either alone or in combination with 7-nitroindazole. Administration of L-arginine before KA attenuated neuronal loss in CA3 but not CA1. A clear protective effect was observed, however, in CA1 and CA3, in rats receiving both L-arginine plus 7-nitroindazole before KA. The results show that the combination of a NO substrate with a NOS inhibitor reduces the neurotoxic effects of KA in the rat hippocampus. This study suggests that extremely fine regulation of NO levels in the different neural cell types can modulate excitotoxicity. (C) 2000 Wiley-Liss, Inc.
Versión del editorhttp://dx.doi.org/10.1002/(SICI)1097-4547(20000315)59:6<797::AID-JNR12>3.0.CO;2-F
Identificadoresdoi: 10.1002/(SICI)1097-4547(20000315)59:6<797::AID-JNR12>3.0.CO;2-F
issn: 0360-4012
Aparece en las colecciones: (IIBB) Artículos
Ficheros en este ítem:
Fichero Descripción Tamaño Formato  
accesoRestringido.pdf15,38 kBAdobe PDFVista previa
Mostrar el registro completo

NOTA: Los ítems de Digital.CSIC están protegidos por copyright, con todos los derechos reservados, a menos que se indique lo contrario.