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Título

IL-4 Expression after Stroke and Alternative Microglia/Macrophage Activation

AutorBonfill-Teixidor, Ester ; Pérez-de-Puig, Isabel ; Salas-Perdomo, Angélica ; Miró-Mur, Francesc; Planas, Anna M.
Fecha de publicación22-oct-2014
CitaciónIX Simposi de Neurobiologia Experimental (2014)
ResumenStroke triggers inflammation that exacerbates brain tissue damage. The inflammatory reaction is naturally set up to clear the necrotic tissue and comprises a complex dynamic process evolving through various steps from initiation to resolution which are not fully elucidated. IL-4 is a cytokine mainly produced by lymphocytes that promotes an alternative anti-inflammatory M2 phenotype in macrophages. Mice deficient in IL-4 show worse stroke outcome suggesting that IL-4 may play a role in brain ischemia. The aim of this study was to identify the IL-4 expression in the brain after ischemia and investigate the effects of IL-4 on the inflammatory response of glial cells. Permanent middle cerebral artery occlusion (pMCAO) in mice produces low levels of IL-4 mRNA expression from 6 hours to 4 days post-ischemia, but it strongly increased at day 7. At this time, the expression of several inflammatory markers was attenuated while that of molecules involved in alternative M2 phenotype and tissue repair increased. Treatment of cultures of murine glia with recombinant IL-4 increasingly upregulated the expression of the M2 markers such as arginase-1 up to 48h while M1 markers are inhibited. This profile in glia was dependent on Jak1/Jak3/Stat6 pathway and requires a new protein synthesis. Microglia treated with IL-4 showed a reduced proinflammatory response when challenged with LPS. We suggest that initial proinflammatory milieu set after brain ischemia is followed by the upregulation of IL-4 and of markers of alternative M2 phenotype and that JAK/STAT pathways are involved. M1 glia can be reprogrammed by IL-4 switching to a M2 phenotype
DescripciónComunicación presentada en el IX Simposi de Neurobiologia Experimental, celebrado los días 22 y 23 de octubre de 2014 en Barcelona y organizado por la Societat Catalana de Biologia del Institut d'Estudis Catalans
URIhttp://hdl.handle.net/10261/116717
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