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Dual-reporter in vivo imaging of transient and inducible heat-shock promoter activation

AutorFortin, PIerre-Yves; Genevois, Coralie; Chapolard, Mathilde; Santalucía, Tomàs ; Planas, Anna M. ; Couillaud, Franck
Fecha de publicación2014
EditorOptical Society of America
CitaciónBiomedical Optics Express 5(2): 457-467 (2014)
ResumenGene promoter activity can be studied in vivo by molecular imaging methods using reporter gene technology. Transcription of the reporter and the reported genes occurs simultaneously. However, imaging depends on reporter protein translation, stability, and cellular fate that may differ among the various proteins. A double transgenic mouse strain expressing the firefly luciferase (lucF) and fluorescent mPlum protein under the transcriptional control of the thermo-inducible heat-shock protein (Hspa1b) promoter was generated allowing to follow up the reporter proteins by different and complementary in vivo imaging technologies. These mice were used for in vivo imaging by bioluminescence and epi fluorescence reflectance imaging (BLI & FRI) and as a source of embryonic fibroblast (MEF) for in vitro approaches. LucF, mPlum and endogenous Hsp70 mRNAs were transcribed simultaneously. The increase in mRNA was transient, peaking at 3 h and then returning to the basal level about 6 h after the thermal stimulations. The bioluminescent signal was transient and initiated with a 3 h delay versus mRNA expression. The onset of mPlum fluorescence was more delayed, increasing slowly up to 30 h after heatshock and remaining for several days. This mouse allows for both bioluminescence imaging (BLI) and fluorescence reflectance imaging (FRI) of Hsp70 promoter activation showing an early and transient lucF activity and a retrospective and persistent mPlum fluorescence. This transgenic mouse will allow following the transient local induction of Hsp-70 promoter beyond its induction time-frame and relate into subsequent dynamic biological effects of the heat-shock response. © 2014 Optical Society of America.
Versión del editorhttp://dx.doi.org/10.1364/BOE.5.000457
Identificadoresdoi: 10.1364/BOE.5.000457
issn: 2156-7085
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