English   español  
Por favor, use este identificador para citar o enlazar a este item: http://hdl.handle.net/10261/116673
Título

Unraveling the specific regulation of the central pathway for anaerobic degradation of 3-methylbenzoate.

AutorJuárez, Javier F. ; Liu, Huixiang; Zamarro, María Teresa ; McMahon, Stephen; Liu, Huanting; Naismith, James H.; Eberlein, C.; Boll, Matthias; Carmona, Manuel ; Díaz, Eduardo
Palabras claveTranscriptional regulation
Azoarcus
Anaerobic degradation
3-methylbenzoate
Fecha de publicación8-may-2015
EditorAmerican Society for Biochemistry and Molecular Biology
CitaciónJ Biol Chem. 290(19):12165-83 (2015)
ResumenThe mbd cluster encodes the anaerobic degradation of 3-methylbenzoate in the β-proteobacterium Azoarcus sp. CIB. The specific transcriptional regulation circuit that controls the expression of the mbd genes was investigated. The PO, PB 1, and P3 R promoters responsible for the expression of the mbd genes, their cognate MbdR transcriptional repressor, as well as the MbdR operator regions (ATACN10GTAT) have been characterized. The three-dimensional structure of MbdR has been solved revealing a conformation similar to that of other TetR family transcriptional regulators. The first intermediate of the catabolic pathway, i.e. 3-methylbenzoyl-CoA, was shown to act as the inducer molecule. An additional MbdR-dependent promoter, PA, which contributes to the expression of the CoA ligase that activates 3-methylbenzoate to 3-methylbenzoyl-CoA, was shown to be necessary for an efficient induction of the mbd genes. Our results suggest that the mbd cluster recruited a regulatory system based on the MbdR regulator and its target promoters to evolve a distinct central catabolic pathway that is only expressed for the anaerobic degradation of aromatic compounds that generate 3-methylbenzoyl-CoA as the central metabolite. All these results highlight the importance of the regulatory systems in the evolution and adaptation of bacteria to the anaerobic degradation of aromatic compounds.
Descripción19 p.-9 fig.-3 tab.
Versión del editorhttp://dx.doi.org/10.1074/jbc.M115.637074
URIhttp://hdl.handle.net/10261/116673
DOI10.1074/jbc.M115.637074
ISSN0021-9258
E-ISSN1083-351X
Aparece en las colecciones: (CIB) Artículos
Ficheros en este ítem:
Fichero Descripción Tamaño Formato  
JBC 2015.pdf5,65 MBAdobe PDFVista previa
Visualizar/Abrir
Mostrar el registro completo
 

Artículos relacionados:


NOTA: Los ítems de Digital.CSIC están protegidos por copyright, con todos los derechos reservados, a menos que se indique lo contrario.