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Cellular viscosity and the stability of low-molecular weight biomolecules under high temperatures

AutorCuecas, Alba ; Cruces Tova, Jorge; Peng, X.; González Grau, Juan Miguel
Fecha de publicación8-sep-2014
EditorInternational Society for Extremophiles
CitaciónBook of abstract of the 10th International Congress on Extremophiles (Extremophiles 2014). Physiology and Genomics, P73 página. 192 (2014)
ResumenLow-molecular weight biomolecules (e. g., nicotinamide nucleotides among others) are essential for cell functioning in all living beings. Nevertheless, these molecules are known to be unstable at high temperatures which could represent a limiting factor for the growth of thermophiles. Viscosity influences kinetic energy of molecules and so it could affect their thermostability. In this study, NADH stability at different temperatures and viscosities was studied. NADH decay increases when temperature increases. If viscosity decreases, NADH decay rate increases becoming increasingly unstable. These results suggest that increasing cellular viscosity could be a strategy to maintain the stability of low-molecular weight biomolecules (i. e., NADH) at high temperatures. Cellular viscosity was determined using a fluorescent molecular rotor in various prokaryotic species covering the growth temperature range from 10ºC to 100ºC. Thermophiles and extreme thermophiles maintained a relatively high cellular viscosity suggesting this strategy as a reasonable mechanism to thrive under high temperatures. Due to the drastic reduction of viscosity at increasing temperatures, hyperthermophiles could require additional alternatives to explain their growth at extremely high temperatures. Assuming the cellular cytoplasm is a complex, highly crowded mixture of molecules, the cell interior could provide higher thermal stability for low-molecular weight biomolecules than previously envisioned in water or diluted solutions. Results substantiate the capability to use unstable low-molecular weight biomolecules by thermophiles and extreme thermophiles although these data keep research open for additional mechanisms to be demonstrated to aid hyperthermophiles to overcome that limitation.
DescripciónPoster presentación en el 10th International Congress on Extremophiles (Extremophiles 2014), September 7-11, 2014, Saint Petersburg, Russia
Versión del editorhttp://www.extremophiles2014.ru/pdf/Book-of-Abstracts-29-09-2014.pdf
Aparece en las colecciones: (IRNAS) Comunicaciones congresos
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