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Título: | miRNA let-7e Modulates the Wnt Pathway and Early Nephrogenic Markers in Mouse Embryonic Stem Cell Differentiation |
Autor: | Viñas, Jose Luis CSIC ORCID; Ventayol, Marina CSIC; Brüne, Bernhard; Jung, Michaela CSIC; Solà, Anna M. CSIC ORCID; Pi, Felip CSIC; Mastora, Chrysoula CSIC; Hotter, Georgina CSIC ORCID | Fecha de publicación: | 10-abr-2013 | Editor: | Public Library of Science | Citación: | PLoS ONE 8(4): e60937 (2013) | Resumen: | This study indicates that embryonic stem cells [ESCs] cultured with retinoic acid and activin A significantly upregulate the miRNA let-7e. This specific miRNA modulates the Wnt pathway and the expression of early nephrogenic markers under these differentiation conditions. The differentiation markers WT1, Pax2 and Wnt4 were downregulated when miRNA let-7e was silenced, thus indicating the role of miRNA let-7e in the differentiation process. PKCβ, GSK3β phosphorylation (GSK3βP) and β-catenin expression was reduced in differentiated cells and reversed by miRNA let-7e silencing. Addition of a PKCβ inhibitor to the miRNA let-7e silenced cells abolished let-7e-derived effects in differentiation markers, and reversed the increase in GSK3βP and β-catenin, thus indicating that miRNA let-7e is involved in differentiation via the modulation of GSK3β phosphorylation and β-catenin production. © 2013 Viñas et al. | Versión del editor: | http://dx.doi.org/10.1371/journal.pone.0060937 | URI: | http://hdl.handle.net/10261/116488 | DOI: | 10.1371/journal.pone.0060937 | Identificadores: | doi: 10.1371/journal.pone.0060937 issn: 1932-6203 |
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