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Title

Low O2 and high CO2 in LLC-PK1 cells culture mimics renal ischemia-induced apoptosis

AuthorsHotter, Georgina ; Palacios, Luis; Solà, Anna M.
KeywordsHypoxia
Kidney
Renal cells
Apoptosis
Hypercapnia
Ischemia
Issue Date15-Dec-2003
PublisherNature Publishing Group
CitationLaboratory Investigation 84(2): 213-220 (2004)
AbstractIschemia, absence or loss of blood flow in organs always presents as a dual phenomenon: tissue oxygen deficit and CO2 excess (hypercapnia). Commonly hypoxic cell culture models kept CO2 at normal nonischemic values. We report a study of apoptosis in an in vitro model of renal hypoxia that mimics in vivo tissue gas atmosphere composition determined during experimental ischemia in rat kidney (low O2 plus high CO 2). Renal tubular LLC-PK1 cell were transiently exposed to hypoxia, to hypercapnia or to both conditions (simulated ischemia). Exposure to simulated ischemic atmosphere, but not to low O2 or high CO2 alone, induced cell apoptosis in vitro. This suggests that ischemia-induced apoptosis in vivo would be dependent on the natural, joint action of hypoxia and hypercapnia. This should be taken into account in cell culture studies that would like to mimic in vivo ischemic conditions. © 2004 USCAP, Inc. All rights reserved.
Publisher version (URL)http://dx.doi.org/10.1038/labinvest.3700026
URIhttp://hdl.handle.net/10261/116487
DOI10.1038/labinvest.3700026
Identifiersdoi: 10.1038/labinvest.3700026
issn: 0023-6837
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