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PBF complex regulates directly Ace2p-target genes in Schizosaccharomyces pombe

AuthorsSuárez, M. Belén CSIC; Alonso-Núñez, María Luisa CSIC; Encinar del Dedo, Javier CSIC ORCID ; McInnerny, Christopher J.; Rey Iglesias, Francisco del; Vázquez de Aldana, Carlos R. CSIC ORCID
Issue Date2013
CitationPombe 2013
AbstractYeast division cycle is completed with the activation of a cell separation program that involves dissolution of the septum assemblied during cytokinesis between the two daughter cells, allowing them to become independent entities. Gene expression of hydrolytic enzymes (eng1+ and agn1+) responsible for septum dissolution is periodically activated at the end of the cell cycle by the specific transcription factor Ace2p (Alonso-Núñez et al., 2005). ace2+ expression is, in turn, periodically activated during mitosis by the transcriptional complex PBF (>PCB Binding Factor>). This complex consists of at least two forkhead-like proteins Sep1p, Fkh2p and a MADS box-like protein Mbx1p, although more recently it has been shown that other proteins participating in different events at the end of the cell cycle, as the kinase Plo1p, the phosphatase Clp1p and the anillin-like protein Mid1p, are also components of PBF (McInerny, JC 2011). In our laboratory, we have found that Ace2p-dependent genes show in their upstream promoter region sites for Ace2p binding but also PCB sites (>Pombe Cell-Cycle Box>) for PBF complex binding. Chromatin immunoprecipitation analyses (ChIP-qPCR) showed that at least Fkh2p and Sep1 bind in vivo to the eng1+ promoter. ChIP analyses through the cell cycle also revealed that Ace2p binding was coincident with maximum level of eng1+ mRNA, while Fkh2p was found to bind later, when mRNA level of this gene is low. Additionally, RT-qPCR assays in ace2Δ, fkh2Δ, sep1Δ, and mbx1Δ mutants show that expression of the genes under the control of Ace2p is affected in a different way by PBF components, indicating that transcriptional regulation is more complex than initially expected.
DescriptionResumen del póster presentado a la EMBO Conference on Fission Yeast: Pombe 2013; 7th International Fission Yeast Meeting celebrada en Londres (UK) del 24 al 29 de junio de 2013.
Appears in Collections:(IBFG) Comunicaciones congresos
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