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Fission yeast Mhf1 (CENP-S) and Mhf2 (CENP-X) play a role in genome stability

AuthorsTormos-Pérez, Marta ; Chica, Nathalia ; Pérez-Hidalgo, Livia ; Botet, Javier ; Moreno, Sergio
Issue Date2013
CitationPombe 2013
AbstractOur laboratory is interested in understanding the complex molecular mechanisms that regulate cell division and its coordination with cell growth and DNA replication in eukaryotes. We have undertaken a functional genomic approach to identify novel cell cycle regulators and gain greater insight into the mechanisms that control cell cycle progression in fission yeast. For this purpose we have used a synthetic lethal approach (SpSGA) to investigate the genetic interactions between the collection of non-essential fission yeast mutants (Bioneer version 3.0) and the query mutant strains wee1-50 and cdc2-3w that have wee phenotypes characterized by a short G2 phase and small cell size. We identified mhf1Δ and mhf2Δ among the 70 mutants that displayed negative genetic interactions with wee1-50 and cdc2-3w. Mhf1 (CENP-S) and Mhf2 (CENP-X) are two histone-fold proteins, highly conserved from yeast to mammalian cells, that play an only partially understood role in the kinetochore architecture and kinetochore-microtubule dynamics. In mammalian cells MHF1 and MHF2 are also involved in DNA repair, specifically in the Fanconi anaemia pathway. For these reasons, we are functionally characterising these proteins in fission yeast. Our preliminary results show that both Mhf1 and Mhf2 are required for proper chromosome segregation during mitosis and meiosis. We have observed that the mutants lacking mhf1+ or mhf2+ show genomic instability and a significant increase in the rate of chromosome loss. We are now beginning to understand the role of these proteins in the maintenance of genome stability during chromosome segregation at cell division and during DNA repair/remodelling processes upon replicative stress.
DescriptionResumen del póster presentado a la EMBO Conference on Fission Yeast: Pombe 2013; 7th International Fission Yeast Meeting celebrada en Londres (UK) del 24 al 29 de junio de 2013.
Appears in Collections:(IBFG) Comunicaciones congresos
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